Abstract
Background: Although a challenge test using non-steroidal anti-inflammatory drugs (NSAIDs) is crucial for diagnosis of aspirin-induced asthma (AIA), it also has drawbacks in terms of possible side effects. Therefore, alternative in-vitro diagnostic methods for AIA are awaited. Methods: Nineteen stable non-AIA patients (9 males and 10 females; mean age, 49.4 ± 4.8 years), and 20 AIA patients (9 males and 11 females; mean age, 51.1 ± 4.8 years) were enrolled in this study. CD11b and CD16 expressions on the peripheral-blood granulocytes after administration of aspirin and different concentrations of PGE2 in vitro were examined using flowcytometry. Results: Aspirin induced a significant increase in CD11b expression on eosinophils (CD16 negative granulocytes) in 19 AIA patients and one non-AIA patient. Increase in CD11b expression on eosinophils by aspirin administration was suppressed by PGE2 in a dose-dependent manner. Conclusions: The measurement of CD11b expression on peripheral-blood eosinophils showed very high sensitivity and specificity of (-95%) in diagnosing AIA. Although this method requires laboratory facilities for flowcytometry, it may be very useful in diagnosis of AIA without side effects. In addition, PGE2 may be involved in regulation of CD11b expression on eosinophils by aspirin administration.
| Original language | English |
|---|---|
| Pages (from-to) | 367-373 |
| Number of pages | 7 |
| Journal | Allergology International |
| Volume | 62 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 01-01-2013 |
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All Science Journal Classification (ASJC) codes
- Immunology and Allergy
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Upregulation of CD11b on eosinophils in aspirin induced asthma. / Isogai, Sumito; Hayashi, Masamichi; Yamamoto, Naoki; Morishita, Mariko; Minezawa, Tomoyuki; Okamura, Takuya; Hoshino, Tami; Okazawa, Mitsushi; Imaizumi, Kazuyoshi.
In: Allergology International, Vol. 62, No. 3, 01.01.2013, p. 367-373.Research output: Contribution to journal › Article
TY - JOUR
T1 - Upregulation of CD11b on eosinophils in aspirin induced asthma
AU - Isogai, Sumito
AU - Hayashi, Masamichi
AU - Yamamoto, Naoki
AU - Morishita, Mariko
AU - Minezawa, Tomoyuki
AU - Okamura, Takuya
AU - Hoshino, Tami
AU - Okazawa, Mitsushi
AU - Imaizumi, Kazuyoshi
PY - 2013/1/1
Y1 - 2013/1/1
N2 - Background: Although a challenge test using non-steroidal anti-inflammatory drugs (NSAIDs) is crucial for diagnosis of aspirin-induced asthma (AIA), it also has drawbacks in terms of possible side effects. Therefore, alternative in-vitro diagnostic methods for AIA are awaited. Methods: Nineteen stable non-AIA patients (9 males and 10 females; mean age, 49.4 ± 4.8 years), and 20 AIA patients (9 males and 11 females; mean age, 51.1 ± 4.8 years) were enrolled in this study. CD11b and CD16 expressions on the peripheral-blood granulocytes after administration of aspirin and different concentrations of PGE2 in vitro were examined using flowcytometry. Results: Aspirin induced a significant increase in CD11b expression on eosinophils (CD16 negative granulocytes) in 19 AIA patients and one non-AIA patient. Increase in CD11b expression on eosinophils by aspirin administration was suppressed by PGE2 in a dose-dependent manner. Conclusions: The measurement of CD11b expression on peripheral-blood eosinophils showed very high sensitivity and specificity of (-95%) in diagnosing AIA. Although this method requires laboratory facilities for flowcytometry, it may be very useful in diagnosis of AIA without side effects. In addition, PGE2 may be involved in regulation of CD11b expression on eosinophils by aspirin administration.
AB - Background: Although a challenge test using non-steroidal anti-inflammatory drugs (NSAIDs) is crucial for diagnosis of aspirin-induced asthma (AIA), it also has drawbacks in terms of possible side effects. Therefore, alternative in-vitro diagnostic methods for AIA are awaited. Methods: Nineteen stable non-AIA patients (9 males and 10 females; mean age, 49.4 ± 4.8 years), and 20 AIA patients (9 males and 11 females; mean age, 51.1 ± 4.8 years) were enrolled in this study. CD11b and CD16 expressions on the peripheral-blood granulocytes after administration of aspirin and different concentrations of PGE2 in vitro were examined using flowcytometry. Results: Aspirin induced a significant increase in CD11b expression on eosinophils (CD16 negative granulocytes) in 19 AIA patients and one non-AIA patient. Increase in CD11b expression on eosinophils by aspirin administration was suppressed by PGE2 in a dose-dependent manner. Conclusions: The measurement of CD11b expression on peripheral-blood eosinophils showed very high sensitivity and specificity of (-95%) in diagnosing AIA. Although this method requires laboratory facilities for flowcytometry, it may be very useful in diagnosis of AIA without side effects. In addition, PGE2 may be involved in regulation of CD11b expression on eosinophils by aspirin administration.
UR - http://www.scopus.com/inward/record.url?scp=84884298686&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84884298686&partnerID=8YFLogxK
U2 - 10.2332/allergolint.12-OA-0499
DO - 10.2332/allergolint.12-OA-0499
M3 - Article
C2 - 23880611
AN - SCOPUS:84884298686
VL - 62
SP - 367
EP - 373
JO - Allergology International
JF - Allergology International
SN - 1323-8930
IS - 3
ER -