Upregulation of ceramide and its regulating mechanism in a rat model of chronic cerebral ischemia

Ryo Ohtani, Hidekazu Tomimoto, Tadakazu Kondo, Hideaki Wakita, Ichiro Akiguchi, Hiroshi Shibasaki, Toshiro Okazaki

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Abstract

Ceramide is a key mediator of apoptosis, and is involved in the cellular stress response. We examined the alterations in the ceramide levels and their synthetic/degradative pathway in a rat model of chronic cerebral ischemia, in which ischemic white matter (WM) lesions occur in association with oligodendroglial cell apoptosis. Chronic cerebral ischemia was induced by clipping both common carotid arteries in male Wistar rats. After predetermined periods of 1, 3, 7 and 14 days, the animals were subjected to immunohistochemical and biochemical investigations for ceramide in the region containing the frontal cortex and corpus callosum (region 1), and the region containing the internal capsule and globus pallidus (region 2). After 14 days, the myelin was degraded in the corpus callosum, internal capsule and the optic tract in Klüver-Barrera staining. There was a significant increase in the ceramide level and the activity of its synthetic enzyme, acidic sphingomyelinase (SMase), whereas its degrading enzyme, glucosylceramide synthase (GCS), was downregulated in both regions 1 and 2 as compared to the sham-operated rats. Simultaneously, ceramide immunoreactive glia increased in number in the corpus callosum and the internal capsule after 3, 7 and 14 days. Double labeling for ceramide with glial fibrillary acidic protein but not with leukocyte common antigen indicated the astroglial nature of these glia. These findings indicate that chronic cerebral ischemia induces an increased ceramide level in astroglia as a result of downregulation of GCS and an upregulation of ASMase activity.

Original languageEnglish
Pages (from-to)31-40
Number of pages10
JournalBrain Research
Volume1023
Issue number1
DOIs
Publication statusPublished - 08-10-2004

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Ceramides
Brain Ischemia
Up-Regulation
ceramide glucosyltransferase
Internal Capsule
Corpus Callosum
Neuroglia
Down-Regulation
Apoptosis
CD45 Antigens
Sphingomyelin Phosphodiesterase
Globus Pallidus
Common Carotid Artery
Glial Fibrillary Acidic Protein
Frontal Lobe
Enzymes
Myelin Sheath
Astrocytes
Wistar Rats
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Ohtani, Ryo ; Tomimoto, Hidekazu ; Kondo, Tadakazu ; Wakita, Hideaki ; Akiguchi, Ichiro ; Shibasaki, Hiroshi ; Okazaki, Toshiro. / Upregulation of ceramide and its regulating mechanism in a rat model of chronic cerebral ischemia. In: Brain Research. 2004 ; Vol. 1023, No. 1. pp. 31-40.
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abstract = "Ceramide is a key mediator of apoptosis, and is involved in the cellular stress response. We examined the alterations in the ceramide levels and their synthetic/degradative pathway in a rat model of chronic cerebral ischemia, in which ischemic white matter (WM) lesions occur in association with oligodendroglial cell apoptosis. Chronic cerebral ischemia was induced by clipping both common carotid arteries in male Wistar rats. After predetermined periods of 1, 3, 7 and 14 days, the animals were subjected to immunohistochemical and biochemical investigations for ceramide in the region containing the frontal cortex and corpus callosum (region 1), and the region containing the internal capsule and globus pallidus (region 2). After 14 days, the myelin was degraded in the corpus callosum, internal capsule and the optic tract in Kl{\"u}ver-Barrera staining. There was a significant increase in the ceramide level and the activity of its synthetic enzyme, acidic sphingomyelinase (SMase), whereas its degrading enzyme, glucosylceramide synthase (GCS), was downregulated in both regions 1 and 2 as compared to the sham-operated rats. Simultaneously, ceramide immunoreactive glia increased in number in the corpus callosum and the internal capsule after 3, 7 and 14 days. Double labeling for ceramide with glial fibrillary acidic protein but not with leukocyte common antigen indicated the astroglial nature of these glia. These findings indicate that chronic cerebral ischemia induces an increased ceramide level in astroglia as a result of downregulation of GCS and an upregulation of ASMase activity.",
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Ohtani, R, Tomimoto, H, Kondo, T, Wakita, H, Akiguchi, I, Shibasaki, H & Okazaki, T 2004, 'Upregulation of ceramide and its regulating mechanism in a rat model of chronic cerebral ischemia', Brain Research, vol. 1023, no. 1, pp. 31-40. https://doi.org/10.1016/j.brainres.2004.07.024

Upregulation of ceramide and its regulating mechanism in a rat model of chronic cerebral ischemia. / Ohtani, Ryo; Tomimoto, Hidekazu; Kondo, Tadakazu; Wakita, Hideaki; Akiguchi, Ichiro; Shibasaki, Hiroshi; Okazaki, Toshiro.

In: Brain Research, Vol. 1023, No. 1, 08.10.2004, p. 31-40.

Research output: Contribution to journalArticle

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