Urinary Neutrophil-Gelatinase Associated Lipocalin is a Potential Noninvasive Marker for Renal Scarring in Patients With Vesicoureteral Reflux

Manabu Ichino, Mamoru Kusaka, Yoko Kuroyanagi, Terumi Mori, Masashi Morooka, Hitomi Sasaki, Ryoichi Shiroki, Seiichirou Shishido, Hiroki Kurahashi, Kiyotaka Hoshinaga

Research output: Contribution to journalArticle

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Abstract

Purpose: Renal scarring is a serious complication that often occurs with chronic pyelonephritis in the presence of vesicoureteral reflux. In a previous study we established a rat model of renal scarring in which we found the up-regulation of neutrophil-gelatinase associated lipocalin at the mRNA and protein levels. In this study we evaluated urinary neutrophil-gelatinase associated lipocalin as a potential biomarker for progression of renal scarring in patients with vesicoureteral reflux. Materials and Methods: A total of 34 patients diagnosed with vesicoureteral reflux without evidence of current urinary tract infection and 28 normal healthy children were enrolled in this study. Renal scars were evaluated by 99mtechnetium dimercapto-succinic acid renal scan in 24 of the reflux cases. Urinary neutrophil-gelatinase associated lipocalin levels were monitored by ELISA. Results: In normal subjects urinary neutrophil-gelatinase associated lipocalin was high during infancy, decreased rapidly within the following year and reached a low stable level from age 3 years onward. Urinary neutrophil-gelatinase associated lipocalin levels, normalized to age matched standards, were significantly increased in patients with vesicoureteral reflux compared to controls. These levels did not correlate with reflux grade, but were significantly higher in patients with radiological evidence of renal scarring irrespective of reflux grade. Conclusions: Estimation of urinary neutrophil-gelatinase associated lipocalin may be useful as a noninvasive diagnostic or prognostic biomarker for renal scarring.

Original languageEnglish
Pages (from-to)2001-2007
Number of pages7
JournalJournal of Urology
Volume183
Issue number5
DOIs
Publication statusPublished - 01-05-2010

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Vesico-Ureteral Reflux
Cicatrix
Kidney
Biomarkers
Pyelonephritis
Succinic Acid
Lipocalin-2
Urinary Tract Infections
Up-Regulation
Enzyme-Linked Immunosorbent Assay
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Urology

Cite this

Ichino, Manabu ; Kusaka, Mamoru ; Kuroyanagi, Yoko ; Mori, Terumi ; Morooka, Masashi ; Sasaki, Hitomi ; Shiroki, Ryoichi ; Shishido, Seiichirou ; Kurahashi, Hiroki ; Hoshinaga, Kiyotaka. / Urinary Neutrophil-Gelatinase Associated Lipocalin is a Potential Noninvasive Marker for Renal Scarring in Patients With Vesicoureteral Reflux. In: Journal of Urology. 2010 ; Vol. 183, No. 5. pp. 2001-2007.
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abstract = "Purpose: Renal scarring is a serious complication that often occurs with chronic pyelonephritis in the presence of vesicoureteral reflux. In a previous study we established a rat model of renal scarring in which we found the up-regulation of neutrophil-gelatinase associated lipocalin at the mRNA and protein levels. In this study we evaluated urinary neutrophil-gelatinase associated lipocalin as a potential biomarker for progression of renal scarring in patients with vesicoureteral reflux. Materials and Methods: A total of 34 patients diagnosed with vesicoureteral reflux without evidence of current urinary tract infection and 28 normal healthy children were enrolled in this study. Renal scars were evaluated by 99mtechnetium dimercapto-succinic acid renal scan in 24 of the reflux cases. Urinary neutrophil-gelatinase associated lipocalin levels were monitored by ELISA. Results: In normal subjects urinary neutrophil-gelatinase associated lipocalin was high during infancy, decreased rapidly within the following year and reached a low stable level from age 3 years onward. Urinary neutrophil-gelatinase associated lipocalin levels, normalized to age matched standards, were significantly increased in patients with vesicoureteral reflux compared to controls. These levels did not correlate with reflux grade, but were significantly higher in patients with radiological evidence of renal scarring irrespective of reflux grade. Conclusions: Estimation of urinary neutrophil-gelatinase associated lipocalin may be useful as a noninvasive diagnostic or prognostic biomarker for renal scarring.",
author = "Manabu Ichino and Mamoru Kusaka and Yoko Kuroyanagi and Terumi Mori and Masashi Morooka and Hitomi Sasaki and Ryoichi Shiroki and Seiichirou Shishido and Hiroki Kurahashi and Kiyotaka Hoshinaga",
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Ichino, M, Kusaka, M, Kuroyanagi, Y, Mori, T, Morooka, M, Sasaki, H, Shiroki, R, Shishido, S, Kurahashi, H & Hoshinaga, K 2010, 'Urinary Neutrophil-Gelatinase Associated Lipocalin is a Potential Noninvasive Marker for Renal Scarring in Patients With Vesicoureteral Reflux', Journal of Urology, vol. 183, no. 5, pp. 2001-2007. https://doi.org/10.1016/j.juro.2010.01.031

Urinary Neutrophil-Gelatinase Associated Lipocalin is a Potential Noninvasive Marker for Renal Scarring in Patients With Vesicoureteral Reflux. / Ichino, Manabu; Kusaka, Mamoru; Kuroyanagi, Yoko; Mori, Terumi; Morooka, Masashi; Sasaki, Hitomi; Shiroki, Ryoichi; Shishido, Seiichirou; Kurahashi, Hiroki; Hoshinaga, Kiyotaka.

In: Journal of Urology, Vol. 183, No. 5, 01.05.2010, p. 2001-2007.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Urinary Neutrophil-Gelatinase Associated Lipocalin is a Potential Noninvasive Marker for Renal Scarring in Patients With Vesicoureteral Reflux

AU - Ichino, Manabu

AU - Kusaka, Mamoru

AU - Kuroyanagi, Yoko

AU - Mori, Terumi

AU - Morooka, Masashi

AU - Sasaki, Hitomi

AU - Shiroki, Ryoichi

AU - Shishido, Seiichirou

AU - Kurahashi, Hiroki

AU - Hoshinaga, Kiyotaka

PY - 2010/5/1

Y1 - 2010/5/1

N2 - Purpose: Renal scarring is a serious complication that often occurs with chronic pyelonephritis in the presence of vesicoureteral reflux. In a previous study we established a rat model of renal scarring in which we found the up-regulation of neutrophil-gelatinase associated lipocalin at the mRNA and protein levels. In this study we evaluated urinary neutrophil-gelatinase associated lipocalin as a potential biomarker for progression of renal scarring in patients with vesicoureteral reflux. Materials and Methods: A total of 34 patients diagnosed with vesicoureteral reflux without evidence of current urinary tract infection and 28 normal healthy children were enrolled in this study. Renal scars were evaluated by 99mtechnetium dimercapto-succinic acid renal scan in 24 of the reflux cases. Urinary neutrophil-gelatinase associated lipocalin levels were monitored by ELISA. Results: In normal subjects urinary neutrophil-gelatinase associated lipocalin was high during infancy, decreased rapidly within the following year and reached a low stable level from age 3 years onward. Urinary neutrophil-gelatinase associated lipocalin levels, normalized to age matched standards, were significantly increased in patients with vesicoureteral reflux compared to controls. These levels did not correlate with reflux grade, but were significantly higher in patients with radiological evidence of renal scarring irrespective of reflux grade. Conclusions: Estimation of urinary neutrophil-gelatinase associated lipocalin may be useful as a noninvasive diagnostic or prognostic biomarker for renal scarring.

AB - Purpose: Renal scarring is a serious complication that often occurs with chronic pyelonephritis in the presence of vesicoureteral reflux. In a previous study we established a rat model of renal scarring in which we found the up-regulation of neutrophil-gelatinase associated lipocalin at the mRNA and protein levels. In this study we evaluated urinary neutrophil-gelatinase associated lipocalin as a potential biomarker for progression of renal scarring in patients with vesicoureteral reflux. Materials and Methods: A total of 34 patients diagnosed with vesicoureteral reflux without evidence of current urinary tract infection and 28 normal healthy children were enrolled in this study. Renal scars were evaluated by 99mtechnetium dimercapto-succinic acid renal scan in 24 of the reflux cases. Urinary neutrophil-gelatinase associated lipocalin levels were monitored by ELISA. Results: In normal subjects urinary neutrophil-gelatinase associated lipocalin was high during infancy, decreased rapidly within the following year and reached a low stable level from age 3 years onward. Urinary neutrophil-gelatinase associated lipocalin levels, normalized to age matched standards, were significantly increased in patients with vesicoureteral reflux compared to controls. These levels did not correlate with reflux grade, but were significantly higher in patients with radiological evidence of renal scarring irrespective of reflux grade. Conclusions: Estimation of urinary neutrophil-gelatinase associated lipocalin may be useful as a noninvasive diagnostic or prognostic biomarker for renal scarring.

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