Utility of the dried blood on filter paper as a source of cytokine mRNA for the analysis of immunoreactions in Plasmodium yoelii infection

Yoshimasa Maeno, Shusuke Nakazawa, Shigeo Nagashima, Jun Sasaki, Kyoko M. Higo, Koki Taniguchi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We examined the utility of dried blood on filter paper for the source of cytokine messenger RNA (mRNA). Total RNA was isolated from the dried blood of mice infected with Plasmodium yoelii, and cDNA was amplified by reverse transcription-polymerase chain reaction (RT-PCR). As a reference, we extracted total RNA from peripheral blood collected at the same time as the preparation for dried blood. There was no difference in cytokine mRNA expression between the two sources; the dried blood on filter paper and the peripheral blood. Th1 cells, Th2 cells, and monocytes/macrophages derived cytokine mRNAs in the dried blood from infected mice were detected, and the increase of some of the cytokines mRNAs after infection was also observed. These results suggested that the dried blood on filter paper is satisfactory RNA source for immunological examination in field-based studies.

Original languageEnglish
Pages (from-to)295-300
Number of pages6
JournalActa Tropica
Volume87
Issue number2
DOIs
Publication statusPublished - 01-07-2003

Fingerprint

Plasmodium yoelii
messenger RNA
Malaria
cytokines
Cytokines
Messenger RNA
blood
infection
RNA
Th2 Cells
Th1 Cells
mice
monocytes
Reverse Transcription
macrophages
Complementary DNA
reverse transcriptase polymerase chain reaction
Macrophages
cells
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Infectious Diseases

Cite this

Maeno, Yoshimasa ; Nakazawa, Shusuke ; Nagashima, Shigeo ; Sasaki, Jun ; Higo, Kyoko M. ; Taniguchi, Koki. / Utility of the dried blood on filter paper as a source of cytokine mRNA for the analysis of immunoreactions in Plasmodium yoelii infection. In: Acta Tropica. 2003 ; Vol. 87, No. 2. pp. 295-300.
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Utility of the dried blood on filter paper as a source of cytokine mRNA for the analysis of immunoreactions in Plasmodium yoelii infection. / Maeno, Yoshimasa; Nakazawa, Shusuke; Nagashima, Shigeo; Sasaki, Jun; Higo, Kyoko M.; Taniguchi, Koki.

In: Acta Tropica, Vol. 87, No. 2, 01.07.2003, p. 295-300.

Research output: Contribution to journalArticle

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