Utility of the one-step nucleic acid amplification assay in sentinel node mapping for early gastric cancer patients

Ayako Shimada, Hiroya Takeuchi, Tomohiko Nishi, Shuhei Mayanagi, Kazumasa Fukuda, Koichi Suda, Rieko Nakamura, Norihito Wada, Hirofumi Kawakubo, Tadaki Nakahara, Kaori Kameyama, Yuko Kitagawa

Research output: Contribution to journalArticle

Abstract

Background: To safely perform minimized gastrectomy based on sentinel node (SN) concept for early gastric cancer patients, intraoperative diagnostic accuracy is indispensable. This study aimed to evaluate the clinical utility of the one-step nucleic acid amplification (OSNA) assay in the intraoperative diagnosis of SN metastasis in early gastric cancer patients compared with that of histopathological examination. Methods: We conducted a prospective study using the OSNA assay for 43 patients with cT1N0M0 gastric cancer undergoing gastrectomy with SN mapping. All the SNs and selected non-SNs were examined by routine histopathological diagnosis, and the OSNA assay. Results: We performed permanent histopathology (PH) in 1732 lymph nodes (LNs) (286 SNs and 1446 non-SNs) obtained from 43 patients. We also evaluated 439 LNs (286 SNs and 153 non-SNs) with the OSNA assay in addition to PH. Intraoperative histopathology (IH) was performed in 214 LNs (213 SNs and 1 non-SN). PH revealed LN metastasis in 6 patients (14%), all of whom showed positive SNs by PH. The diagnostic accuracy to predict the LN status based on the SN concept by histological examination was 100%. The concordance rate between the OSNA assay and the PH and IH were 0.970 and 0.981 respectively. Discordant results between PH and OSNA assay were observed in 13 LNs. The sensitivity and specificity of the OSNA assay compared with those of PH were 0.636, and 0.988, and compared with those of IH were 0.800, and 0.995. Conclusion: Our results suggest that the OSNA assay is a useful and convenient tool for the intraoperative detection of SN metastasis in early gastric cancer patients.

Original languageEnglish
Pages (from-to)418-425
Number of pages8
JournalGastric Cancer
Volume23
Issue number3
DOIs
Publication statusPublished - 01-05-2020

All Science Journal Classification (ASJC) codes

  • Oncology
  • Gastroenterology
  • Cancer Research

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    Shimada, A., Takeuchi, H., Nishi, T., Mayanagi, S., Fukuda, K., Suda, K., Nakamura, R., Wada, N., Kawakubo, H., Nakahara, T., Kameyama, K., & Kitagawa, Y. (2020). Utility of the one-step nucleic acid amplification assay in sentinel node mapping for early gastric cancer patients. Gastric Cancer, 23(3), 418-425. https://doi.org/10.1007/s10120-019-01016-9