Utility of Y-STR haplotype and mtDNA sequence in personal identification of human remains

Hiroyoshi Koyama, Mineo Iwasa, Tsukasa Tsuchimochi, Yoshitaka Maeno, Ichiro Isobe, Tomohiro Matsumoto, Masataka Nagao

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The utility of Y-STR haplotype and mtDNA sequence in personal identification from human remains is demonstrated. In five cases, severely damaged human remains were identified by Y-chromosomal short tandem repeat (STR) (Y-STR) haplotyping and mitochondrial DNA (mtDNA) sequencing. DNA was extracted from highly adipoceratous tissues using the phenolchloroform method and polymerase chain reaction amplified for Y-STR haplotyping and sequencing of two hypervariable regions, HV1 and HV2, of the mtDNA. The authors also profiled the Y-STR haplotype and mtDNA sequence of a blood sample that was obtained from the presumptive brother of the decedent. Y-STR haplotyping and mtDNA sequencing results were compared with one another and with the Anderson sequence. It was concluded from these data that the lower part of the body (Case 1), the human head (Case 2), the upper part of the body (Case 3), and the right arm (Case 4) were from the same individual, whereas another lower part of a body (Case 5) was from a relative of the presumptive brother. A novel descriptive way of a statistical characteristic, gene or haplotype diversity, is shown.

Original languageEnglish
Pages (from-to)181-185
Number of pages5
JournalAmerican Journal of Forensic Medicine and Pathology
Volume23
Issue number2
DOIs
Publication statusPublished - 11-06-2002
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

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