Vaccination with FimH adhesin protects cynomolgus monkeys from colonization and infection by uropathogenic Escherichia coli

Solomon Langermann; Roland Möllby; Jeanne E. Burlein; Susan R. Palaszynski; C. Gale Auguste; Anthony DeFusco; Robert Strouse; Mark A. Schenerman; Scott J. Hultgren; Jerome S. Pinkner; Jan Winberg; Lena Guldevall; Mats Söderhäll; Kiyohito Ishikawa; Staffan Normark; Scott Koenig

doi:10.1086/315258

Vaccination with FimH adhesin protects cynomolgus monkeys from colonization and infection by uropathogenic Escherichia coli

Solomon Langermann, Roland Möllby, Jeanne E. Burlein, Susan R. Palaszynski, C. Gale Auguste, Anthony DeFusco, Robert Strouse, Mark A. Schenerman, Scott J. Hultgren, Jerome S. Pinkner, Jan Winberg, Lena Guldevall, Mats Söderhäll, Kiyohito Ishikawa, Staffan Normark, Scott Koenig

Research output: Contribution to journal › Article › peer-review

281 Citations (Scopus)

Abstract

Escherichia coli FimH adhesin mediates binding to the bladder mucosa. In mice, a FimH vaccine protects against bacterial challenge. In this study, 4 monkeys were inoculated with 100 μg of FimCH adhesin-chaperone complex mixed with MF59 adjuvant, and 4 monkeys were given adjuvant only intramuscularly. After 2 doses (day 0 and week 4), a booster at 48 weeks elicited a strong IgG antibody response to FimH in the vaccinated monkeys. All 8 monkeys were challenged with 1 mL of 10⁸ E. coli cystitis isolate NU14. Three of the 4 vaccinated monkeys were protected from bacteruria and pyuria; all control monkeys were infected. These findings suggest that a vaccine based on the FimH adhesin of E. coli type 1 pili may have utility in preventing cystitis in humans.

Original language	English
Pages (from-to)	774-778
Number of pages	5
Journal	Journal of Infectious Diseases
Volume	181
Issue number	2
DOIs	https://doi.org/10.1086/315258
Publication status	Published - 2000
Externally published	Yes

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1086/315258

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Langermann, S., Möllby, R., Burlein, J. E., Palaszynski, S. R., Auguste, C. G., DeFusco, A., Strouse, R., Schenerman, M. A., Hultgren, S. J., Pinkner, J. S., Winberg, J., Guldevall, L., Söderhäll, M., Ishikawa, K., Normark, S., & Koenig, S. (2000). Vaccination with FimH adhesin protects cynomolgus monkeys from colonization and infection by uropathogenic Escherichia coli. Journal of Infectious Diseases, 181(2), 774-778. https://doi.org/10.1086/315258

@article{8ff3c2053ef54f3ab9d8b65ff15e6c5c,

title = "Vaccination with FimH adhesin protects cynomolgus monkeys from colonization and infection by uropathogenic Escherichia coli",

abstract = "Escherichia coli FimH adhesin mediates binding to the bladder mucosa. In mice, a FimH vaccine protects against bacterial challenge. In this study, 4 monkeys were inoculated with 100 μg of FimCH adhesin-chaperone complex mixed with MF59 adjuvant, and 4 monkeys were given adjuvant only intramuscularly. After 2 doses (day 0 and week 4), a booster at 48 weeks elicited a strong IgG antibody response to FimH in the vaccinated monkeys. All 8 monkeys were challenged with 1 mL of 108 E. coli cystitis isolate NU14. Three of the 4 vaccinated monkeys were protected from bacteruria and pyuria; all control monkeys were infected. These findings suggest that a vaccine based on the FimH adhesin of E. coli type 1 pili may have utility in preventing cystitis in humans.",

author = "Solomon Langermann and Roland M{\"o}llby and Burlein, {Jeanne E.} and Palaszynski, {Susan R.} and Auguste, {C. Gale} and Anthony DeFusco and Robert Strouse and Schenerman, {Mark A.} and Hultgren, {Scott J.} and Pinkner, {Jerome S.} and Jan Winberg and Lena Guldevall and Mats S{\"o}derh{\"a}ll and Kiyohito Ishikawa and Staffan Normark and Scott Koenig",

note = "Funding Information: Received 6 July 1999; revised 22 October 1999; electronically published 9 February 2000. All experiments were approved by the local ethics committee for animal experiments. S.J.H. and Washington University have a financial interest in Med-Immune. Financial support: NIH (grants AI-29549, DK-51406); Karolinska Institute research fund (to R.M.). Reprints or correspondence: Dr. Solomon Langermann, MedImmune, Inc., 35 W. Watkins Mill Rd., Gaithersburg, MD 20878 (langermanns @medimmune.com).",

year = "2000",

doi = "10.1086/315258",

language = "English",

volume = "181",

pages = "774--778",

journal = "Journal of Infectious Diseases",

issn = "0022-1899",

publisher = "Oxford University Press",

number = "2",

}

Langermann, S, Möllby, R, Burlein, JE, Palaszynski, SR, Auguste, CG, DeFusco, A, Strouse, R, Schenerman, MA, Hultgren, SJ, Pinkner, JS, Winberg, J, Guldevall, L, Söderhäll, M, Ishikawa, K, Normark, S & Koenig, S 2000, 'Vaccination with FimH adhesin protects cynomolgus monkeys from colonization and infection by uropathogenic Escherichia coli', Journal of Infectious Diseases, vol. 181, no. 2, pp. 774-778. https://doi.org/10.1086/315258

TY - JOUR

T1 - Vaccination with FimH adhesin protects cynomolgus monkeys from colonization and infection by uropathogenic Escherichia coli

AU - Langermann, Solomon

AU - Möllby, Roland

AU - Burlein, Jeanne E.

AU - Palaszynski, Susan R.

AU - Auguste, C. Gale

AU - DeFusco, Anthony

AU - Strouse, Robert

AU - Schenerman, Mark A.

AU - Hultgren, Scott J.

AU - Pinkner, Jerome S.

AU - Winberg, Jan

AU - Guldevall, Lena

AU - Söderhäll, Mats

AU - Ishikawa, Kiyohito

AU - Normark, Staffan

AU - Koenig, Scott

N1 - Funding Information: Received 6 July 1999; revised 22 October 1999; electronically published 9 February 2000. All experiments were approved by the local ethics committee for animal experiments. S.J.H. and Washington University have a financial interest in Med-Immune. Financial support: NIH (grants AI-29549, DK-51406); Karolinska Institute research fund (to R.M.). Reprints or correspondence: Dr. Solomon Langermann, MedImmune, Inc., 35 W. Watkins Mill Rd., Gaithersburg, MD 20878 (langermanns @medimmune.com).

PY - 2000

Y1 - 2000

N2 - Escherichia coli FimH adhesin mediates binding to the bladder mucosa. In mice, a FimH vaccine protects against bacterial challenge. In this study, 4 monkeys were inoculated with 100 μg of FimCH adhesin-chaperone complex mixed with MF59 adjuvant, and 4 monkeys were given adjuvant only intramuscularly. After 2 doses (day 0 and week 4), a booster at 48 weeks elicited a strong IgG antibody response to FimH in the vaccinated monkeys. All 8 monkeys were challenged with 1 mL of 108 E. coli cystitis isolate NU14. Three of the 4 vaccinated monkeys were protected from bacteruria and pyuria; all control monkeys were infected. These findings suggest that a vaccine based on the FimH adhesin of E. coli type 1 pili may have utility in preventing cystitis in humans.

AB - Escherichia coli FimH adhesin mediates binding to the bladder mucosa. In mice, a FimH vaccine protects against bacterial challenge. In this study, 4 monkeys were inoculated with 100 μg of FimCH adhesin-chaperone complex mixed with MF59 adjuvant, and 4 monkeys were given adjuvant only intramuscularly. After 2 doses (day 0 and week 4), a booster at 48 weeks elicited a strong IgG antibody response to FimH in the vaccinated monkeys. All 8 monkeys were challenged with 1 mL of 108 E. coli cystitis isolate NU14. Three of the 4 vaccinated monkeys were protected from bacteruria and pyuria; all control monkeys were infected. These findings suggest that a vaccine based on the FimH adhesin of E. coli type 1 pili may have utility in preventing cystitis in humans.

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DO - 10.1086/315258

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C2 - 10669375

AN - SCOPUS:0034093337

SN - 0022-1899

VL - 181

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EP - 778

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

IS - 2

ER -

Vaccination with FimH adhesin protects cynomolgus monkeys from colonization and infection by uropathogenic Escherichia coli

Abstract

All Science Journal Classification (ASJC) codes

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