Vaccination with FimH adhesin protects cynomolgus monkeys from colonization and infection by uropathogenic Escherichia coli

Solomon Langermann, Roland Möllby, Jeanne E. Burlein, Susan R. Palaszynski, C. Gale Auguste, Anthony DeFusco, Robert Strouse, Mark A. Schenerman, Scott J. Hultgren, Jerome S. Pinkner, Jan Winberg, Lena Guldevall, Mats Söderhäll, Kiyohito Ishikawa, Staffan Normark, Scott Koenig

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231 Citations (Scopus)

Abstract

Escherichia coli FimH adhesin mediates binding to the bladder mucosa. In mice, a FimH vaccine protects against bacterial challenge. In this study, 4 monkeys were inoculated with 100 μg of FimCH adhesin-chaperone complex mixed with MF59 adjuvant, and 4 monkeys were given adjuvant only intramuscularly. After 2 doses (day 0 and week 4), a booster at 48 weeks elicited a strong IgG antibody response to FimH in the vaccinated monkeys. All 8 monkeys were challenged with 1 mL of 108 E. coli cystitis isolate NU14. Three of the 4 vaccinated monkeys were protected from bacteruria and pyuria; all control monkeys were infected. These findings suggest that a vaccine based on the FimH adhesin of E. coli type 1 pili may have utility in preventing cystitis in humans.

Original languageEnglish
Pages (from-to)774-778
Number of pages5
JournalJournal of Infectious Diseases
Volume181
Issue number2
DOIs
Publication statusPublished - 15-03-2000
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Infectious Diseases

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    Langermann, S., Möllby, R., Burlein, J. E., Palaszynski, S. R., Auguste, C. G., DeFusco, A., Strouse, R., Schenerman, M. A., Hultgren, S. J., Pinkner, J. S., Winberg, J., Guldevall, L., Söderhäll, M., Ishikawa, K., Normark, S., & Koenig, S. (2000). Vaccination with FimH adhesin protects cynomolgus monkeys from colonization and infection by uropathogenic Escherichia coli. Journal of Infectious Diseases, 181(2), 774-778. https://doi.org/10.1086/315258