Validation of endoscopic 13C-urea breath test with nondispersive infrared spectrometric analysis in the management of Helicobacter pylori infection

Hajime Isomoto, Ken Ichiro Inoue, Yohei Mizuta, Mio Nakazato, Yusei Kanazawa, Hitoshi Nishiyama, Hiroshi Ohara, Megumi Urata, Katsuhisa Omagari, Masanobu Miyazaki, Kunihiko Murase, Ikuo Murata, Shigeru Kohno

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background/Aims: 13C-EUBT (endoscopic 13C-urea breath test), that combines the conventional urea breath test with endoscopy, provides excellent accuracy for detection of H. pylori. Recently, a simpler, less expensive and isotope-selected nondispersive infrared spectrometer has been developed for the urea breath test. We validated the diagnostic performance of 13C-EUBT using nondispersive infrared spectrometer in the management of H. pylori infection. Methodology: EUBT was performed in 232 patients by first collecting a baseline breath sample followed by upper gastrointestinal endoscopy. A 20-mL aliquot of 13C-urea solution was sprayed over the entire stomach under endoscopic guidance. Breath samples taken 0 and 20 minutes after spraying were analyzed using nondispersive infrared spectrometer. H. pylori infection was assessed by rapid urease test and histology. The cutoff level was determined by a receiver-operating characteristic curve analysis. Forty-four samples were also analyzed by the conventional isotope ratio mass spectrometer to compare results from both analyzers. We also applied the nondispersive infrared spectrometer-based EUBT to evaluate the efficacy of eradication therapy. Results: Employing 2.4 per mL as the best cutoff value, the EUBT yielded an excellent diagnostic accuracy, with a sensitivity of 99% and specificity of 99%. The sensitivity and specificity of the test for assessing eradication therapy were 94% (16/17) and 100% (57/57), respectively. There was a high linear correlation between nondispersive infrared spectrometer and isotope ratio mass spectrometer (r=0.998, p<0.0001). Conclusions: 13C-EUBT using nondispersive infrared spectrometer is a highly accurate and rapid method for the assessment of H. pylori eradication as well as for detecting H. pylori infection. We believe that nondispersive infrared spectrometer gives more rapid and less expensive method for the management of H. pylori infection with the EUBT.

Original languageEnglish
Pages (from-to)422-425
Number of pages4
JournalHepato-Gastroenterology
Volume50
Issue number50
Publication statusPublished - 01-03-2003
Externally publishedYes

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Breath Tests
Helicobacter Infections
Helicobacter pylori
Urea
Isotopes
Sensitivity and Specificity
Gastrointestinal Endoscopy
Urease
ROC Curve
Endoscopy
Stomach
Histology
Therapeutics

All Science Journal Classification (ASJC) codes

  • Hepatology
  • Gastroenterology

Cite this

Isomoto, H., Inoue, K. I., Mizuta, Y., Nakazato, M., Kanazawa, Y., Nishiyama, H., ... Kohno, S. (2003). Validation of endoscopic 13C-urea breath test with nondispersive infrared spectrometric analysis in the management of Helicobacter pylori infection. Hepato-Gastroenterology, 50(50), 422-425.
Isomoto, Hajime ; Inoue, Ken Ichiro ; Mizuta, Yohei ; Nakazato, Mio ; Kanazawa, Yusei ; Nishiyama, Hitoshi ; Ohara, Hiroshi ; Urata, Megumi ; Omagari, Katsuhisa ; Miyazaki, Masanobu ; Murase, Kunihiko ; Murata, Ikuo ; Kohno, Shigeru. / Validation of endoscopic 13C-urea breath test with nondispersive infrared spectrometric analysis in the management of Helicobacter pylori infection. In: Hepato-Gastroenterology. 2003 ; Vol. 50, No. 50. pp. 422-425.
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abstract = "Background/Aims: 13C-EUBT (endoscopic 13C-urea breath test), that combines the conventional urea breath test with endoscopy, provides excellent accuracy for detection of H. pylori. Recently, a simpler, less expensive and isotope-selected nondispersive infrared spectrometer has been developed for the urea breath test. We validated the diagnostic performance of 13C-EUBT using nondispersive infrared spectrometer in the management of H. pylori infection. Methodology: EUBT was performed in 232 patients by first collecting a baseline breath sample followed by upper gastrointestinal endoscopy. A 20-mL aliquot of 13C-urea solution was sprayed over the entire stomach under endoscopic guidance. Breath samples taken 0 and 20 minutes after spraying were analyzed using nondispersive infrared spectrometer. H. pylori infection was assessed by rapid urease test and histology. The cutoff level was determined by a receiver-operating characteristic curve analysis. Forty-four samples were also analyzed by the conventional isotope ratio mass spectrometer to compare results from both analyzers. We also applied the nondispersive infrared spectrometer-based EUBT to evaluate the efficacy of eradication therapy. Results: Employing 2.4 per mL as the best cutoff value, the EUBT yielded an excellent diagnostic accuracy, with a sensitivity of 99{\%} and specificity of 99{\%}. The sensitivity and specificity of the test for assessing eradication therapy were 94{\%} (16/17) and 100{\%} (57/57), respectively. There was a high linear correlation between nondispersive infrared spectrometer and isotope ratio mass spectrometer (r=0.998, p<0.0001). Conclusions: 13C-EUBT using nondispersive infrared spectrometer is a highly accurate and rapid method for the assessment of H. pylori eradication as well as for detecting H. pylori infection. We believe that nondispersive infrared spectrometer gives more rapid and less expensive method for the management of H. pylori infection with the EUBT.",
author = "Hajime Isomoto and Inoue, {Ken Ichiro} and Yohei Mizuta and Mio Nakazato and Yusei Kanazawa and Hitoshi Nishiyama and Hiroshi Ohara and Megumi Urata and Katsuhisa Omagari and Masanobu Miyazaki and Kunihiko Murase and Ikuo Murata and Shigeru Kohno",
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Isomoto, H, Inoue, KI, Mizuta, Y, Nakazato, M, Kanazawa, Y, Nishiyama, H, Ohara, H, Urata, M, Omagari, K, Miyazaki, M, Murase, K, Murata, I & Kohno, S 2003, 'Validation of endoscopic 13C-urea breath test with nondispersive infrared spectrometric analysis in the management of Helicobacter pylori infection', Hepato-Gastroenterology, vol. 50, no. 50, pp. 422-425.

Validation of endoscopic 13C-urea breath test with nondispersive infrared spectrometric analysis in the management of Helicobacter pylori infection. / Isomoto, Hajime; Inoue, Ken Ichiro; Mizuta, Yohei; Nakazato, Mio; Kanazawa, Yusei; Nishiyama, Hitoshi; Ohara, Hiroshi; Urata, Megumi; Omagari, Katsuhisa; Miyazaki, Masanobu; Murase, Kunihiko; Murata, Ikuo; Kohno, Shigeru.

In: Hepato-Gastroenterology, Vol. 50, No. 50, 01.03.2003, p. 422-425.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Validation of endoscopic 13C-urea breath test with nondispersive infrared spectrometric analysis in the management of Helicobacter pylori infection

AU - Isomoto, Hajime

AU - Inoue, Ken Ichiro

AU - Mizuta, Yohei

AU - Nakazato, Mio

AU - Kanazawa, Yusei

AU - Nishiyama, Hitoshi

AU - Ohara, Hiroshi

AU - Urata, Megumi

AU - Omagari, Katsuhisa

AU - Miyazaki, Masanobu

AU - Murase, Kunihiko

AU - Murata, Ikuo

AU - Kohno, Shigeru

PY - 2003/3/1

Y1 - 2003/3/1

N2 - Background/Aims: 13C-EUBT (endoscopic 13C-urea breath test), that combines the conventional urea breath test with endoscopy, provides excellent accuracy for detection of H. pylori. Recently, a simpler, less expensive and isotope-selected nondispersive infrared spectrometer has been developed for the urea breath test. We validated the diagnostic performance of 13C-EUBT using nondispersive infrared spectrometer in the management of H. pylori infection. Methodology: EUBT was performed in 232 patients by first collecting a baseline breath sample followed by upper gastrointestinal endoscopy. A 20-mL aliquot of 13C-urea solution was sprayed over the entire stomach under endoscopic guidance. Breath samples taken 0 and 20 minutes after spraying were analyzed using nondispersive infrared spectrometer. H. pylori infection was assessed by rapid urease test and histology. The cutoff level was determined by a receiver-operating characteristic curve analysis. Forty-four samples were also analyzed by the conventional isotope ratio mass spectrometer to compare results from both analyzers. We also applied the nondispersive infrared spectrometer-based EUBT to evaluate the efficacy of eradication therapy. Results: Employing 2.4 per mL as the best cutoff value, the EUBT yielded an excellent diagnostic accuracy, with a sensitivity of 99% and specificity of 99%. The sensitivity and specificity of the test for assessing eradication therapy were 94% (16/17) and 100% (57/57), respectively. There was a high linear correlation between nondispersive infrared spectrometer and isotope ratio mass spectrometer (r=0.998, p<0.0001). Conclusions: 13C-EUBT using nondispersive infrared spectrometer is a highly accurate and rapid method for the assessment of H. pylori eradication as well as for detecting H. pylori infection. We believe that nondispersive infrared spectrometer gives more rapid and less expensive method for the management of H. pylori infection with the EUBT.

AB - Background/Aims: 13C-EUBT (endoscopic 13C-urea breath test), that combines the conventional urea breath test with endoscopy, provides excellent accuracy for detection of H. pylori. Recently, a simpler, less expensive and isotope-selected nondispersive infrared spectrometer has been developed for the urea breath test. We validated the diagnostic performance of 13C-EUBT using nondispersive infrared spectrometer in the management of H. pylori infection. Methodology: EUBT was performed in 232 patients by first collecting a baseline breath sample followed by upper gastrointestinal endoscopy. A 20-mL aliquot of 13C-urea solution was sprayed over the entire stomach under endoscopic guidance. Breath samples taken 0 and 20 minutes after spraying were analyzed using nondispersive infrared spectrometer. H. pylori infection was assessed by rapid urease test and histology. The cutoff level was determined by a receiver-operating characteristic curve analysis. Forty-four samples were also analyzed by the conventional isotope ratio mass spectrometer to compare results from both analyzers. We also applied the nondispersive infrared spectrometer-based EUBT to evaluate the efficacy of eradication therapy. Results: Employing 2.4 per mL as the best cutoff value, the EUBT yielded an excellent diagnostic accuracy, with a sensitivity of 99% and specificity of 99%. The sensitivity and specificity of the test for assessing eradication therapy were 94% (16/17) and 100% (57/57), respectively. There was a high linear correlation between nondispersive infrared spectrometer and isotope ratio mass spectrometer (r=0.998, p<0.0001). Conclusions: 13C-EUBT using nondispersive infrared spectrometer is a highly accurate and rapid method for the assessment of H. pylori eradication as well as for detecting H. pylori infection. We believe that nondispersive infrared spectrometer gives more rapid and less expensive method for the management of H. pylori infection with the EUBT.

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