Vangl2 regulates E-Cadherin in epithelial cells

Tadahiro Nagaoka, Ayumu Inutsuka, Khadiza Begum, Khandakar Musabbir Bin Hafiz, Masashi Kishi

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

E-cadherin belongs to the classic cadherin subfamily of calcium-dependent cell adhesion molecules and is crucial for the formation and function of epithelial adherens junctions. In this study, we demonstrate that Vangl2, a vertebrate regulator of planar cell polarity (PCP), controls E-cadherin in epithelial cells. E-cadherin co-immunoprecipitates with Vangl2 from embryonic kidney extracts, and this association is also observed in transfected fibroblasts. Vangl2 enhances the internalization of E-cadherin when overexpressed. Conversely, the quantitative ratio of E-cadherin exposed to the cell surface is increased in cultured renal epithelial cells derived from Vangl2Lpt/+ mutant mice. Interestingly, Vangl2 is also internalized through protein traffic involving Rab5- and Dynamin-dependent endocytosis. Taken together with recent reports regarding the transport of Frizzled3, MMP14 and nephrin, these results suggest that one of the molecular functions of Vangl2 is to enhance the internalization of specific plasma membrane proteins with broad selectivity. This function may be involved in the control of intercellular PCP signalling or in the PCP-related rearrangement of cell adhesions.

Original languageEnglish
Article number6940
JournalScientific Reports
Volume4
DOIs
Publication statusPublished - 06-11-2014
Externally publishedYes

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Cadherins
Epithelial Cells
Cell Polarity
Dynamins
Kidney
Adherens Junctions
Cell Adhesion Molecules
Endocytosis
Cell Adhesion
Vertebrates
Blood Proteins
Membrane Proteins
Fibroblasts
Cell Membrane
Calcium
Proteins

All Science Journal Classification (ASJC) codes

  • General

Cite this

Nagaoka, T., Inutsuka, A., Begum, K., Musabbir Bin Hafiz, K., & Kishi, M. (2014). Vangl2 regulates E-Cadherin in epithelial cells. Scientific Reports, 4, [6940]. https://doi.org/10.1038/srep06940
Nagaoka, Tadahiro ; Inutsuka, Ayumu ; Begum, Khadiza ; Musabbir Bin Hafiz, Khandakar ; Kishi, Masashi. / Vangl2 regulates E-Cadherin in epithelial cells. In: Scientific Reports. 2014 ; Vol. 4.
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Nagaoka, T, Inutsuka, A, Begum, K, Musabbir Bin Hafiz, K & Kishi, M 2014, 'Vangl2 regulates E-Cadherin in epithelial cells', Scientific Reports, vol. 4, 6940. https://doi.org/10.1038/srep06940

Vangl2 regulates E-Cadherin in epithelial cells. / Nagaoka, Tadahiro; Inutsuka, Ayumu; Begum, Khadiza; Musabbir Bin Hafiz, Khandakar; Kishi, Masashi.

In: Scientific Reports, Vol. 4, 6940, 06.11.2014.

Research output: Contribution to journalArticle

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Nagaoka T, Inutsuka A, Begum K, Musabbir Bin Hafiz K, Kishi M. Vangl2 regulates E-Cadherin in epithelial cells. Scientific Reports. 2014 Nov 6;4. 6940. https://doi.org/10.1038/srep06940