Vasopressin-secreting neurons derived from human embryonic stem cells through specific induction of dorsal hypothalamic progenitors

Koichiro Ogawa, Hidetaka Suga, Chikafumi Ozone, Mayu Sakakibara, Tomiko Yamada, Mayuko Kano, Kazuki Mitsumoto, Takatoshi Kasai, Yu Kodani, Hiroshi Nagasaki, Naoki Yamamoto, Daisuke Hagiwara, Motomitsu Goto, Ryoichi Banno, Yoshihisa Sugimura, Hiroshi Arima

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Arginine-vasopressin (AVP) neurons exist in the hypothalamus, a major region of the diencephalon, and play an essential role in water balance. Here, we established the differentiation method for AVP-secreting neurons from human embryonic stem cells (hESCs) by recapitulating in vitro the in vivo embryonic developmental processes of AVP neurons. At first, the differentiation efficiency was improved. That was achieved through the optimization of the culture condition for obtaining dorsal hypothalamic progenitors. Secondly, the induced AVP neurons were identified by immunohistochemistry and these neurons secreted AVP after potassium chloride stimulation. Additionally, other hypothalamic neuropeptides were also detected, such as oxytocin, corticotropin-releasing hormone, thyrotropin-releasing hormone, pro-opiomelanocortin, agouti-related peptide, orexin, and melanin-concentrating hormone. This is the first report describing the generation of secretory AVP neurons derived from hESCs. This method will be applicable to research using disease models and, potentially, for regenerative medicine of the hypothalamus.

Original languageEnglish
Article number3615
JournalScientific reports
Volume8
Issue number1
DOIs
Publication statusPublished - 01-12-2018

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Arginine Vasopressin
Vasopressins
Neurons
Hypothalamus
Diencephalon
Pro-Opiomelanocortin
Regenerative Medicine
Thyrotropin-Releasing Hormone
Potassium Chloride
Corticotropin-Releasing Hormone
Oxytocin
Neuropeptides
Human Embryonic Stem Cells
Immunohistochemistry
Peptides
Water
Research

All Science Journal Classification (ASJC) codes

  • General

Cite this

Ogawa, Koichiro ; Suga, Hidetaka ; Ozone, Chikafumi ; Sakakibara, Mayu ; Yamada, Tomiko ; Kano, Mayuko ; Mitsumoto, Kazuki ; Kasai, Takatoshi ; Kodani, Yu ; Nagasaki, Hiroshi ; Yamamoto, Naoki ; Hagiwara, Daisuke ; Goto, Motomitsu ; Banno, Ryoichi ; Sugimura, Yoshihisa ; Arima, Hiroshi. / Vasopressin-secreting neurons derived from human embryonic stem cells through specific induction of dorsal hypothalamic progenitors. In: Scientific reports. 2018 ; Vol. 8, No. 1.
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abstract = "Arginine-vasopressin (AVP) neurons exist in the hypothalamus, a major region of the diencephalon, and play an essential role in water balance. Here, we established the differentiation method for AVP-secreting neurons from human embryonic stem cells (hESCs) by recapitulating in vitro the in vivo embryonic developmental processes of AVP neurons. At first, the differentiation efficiency was improved. That was achieved through the optimization of the culture condition for obtaining dorsal hypothalamic progenitors. Secondly, the induced AVP neurons were identified by immunohistochemistry and these neurons secreted AVP after potassium chloride stimulation. Additionally, other hypothalamic neuropeptides were also detected, such as oxytocin, corticotropin-releasing hormone, thyrotropin-releasing hormone, pro-opiomelanocortin, agouti-related peptide, orexin, and melanin-concentrating hormone. This is the first report describing the generation of secretory AVP neurons derived from hESCs. This method will be applicable to research using disease models and, potentially, for regenerative medicine of the hypothalamus.",
author = "Koichiro Ogawa and Hidetaka Suga and Chikafumi Ozone and Mayu Sakakibara and Tomiko Yamada and Mayuko Kano and Kazuki Mitsumoto and Takatoshi Kasai and Yu Kodani and Hiroshi Nagasaki and Naoki Yamamoto and Daisuke Hagiwara and Motomitsu Goto and Ryoichi Banno and Yoshihisa Sugimura and Hiroshi Arima",
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Ogawa, K, Suga, H, Ozone, C, Sakakibara, M, Yamada, T, Kano, M, Mitsumoto, K, Kasai, T, Kodani, Y, Nagasaki, H, Yamamoto, N, Hagiwara, D, Goto, M, Banno, R, Sugimura, Y & Arima, H 2018, 'Vasopressin-secreting neurons derived from human embryonic stem cells through specific induction of dorsal hypothalamic progenitors', Scientific reports, vol. 8, no. 1, 3615. https://doi.org/10.1038/s41598-018-22053-x

Vasopressin-secreting neurons derived from human embryonic stem cells through specific induction of dorsal hypothalamic progenitors. / Ogawa, Koichiro; Suga, Hidetaka; Ozone, Chikafumi; Sakakibara, Mayu; Yamada, Tomiko; Kano, Mayuko; Mitsumoto, Kazuki; Kasai, Takatoshi; Kodani, Yu; Nagasaki, Hiroshi; Yamamoto, Naoki; Hagiwara, Daisuke; Goto, Motomitsu; Banno, Ryoichi; Sugimura, Yoshihisa; Arima, Hiroshi.

In: Scientific reports, Vol. 8, No. 1, 3615, 01.12.2018.

Research output: Contribution to journalArticle

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T1 - Vasopressin-secreting neurons derived from human embryonic stem cells through specific induction of dorsal hypothalamic progenitors

AU - Ogawa, Koichiro

AU - Suga, Hidetaka

AU - Ozone, Chikafumi

AU - Sakakibara, Mayu

AU - Yamada, Tomiko

AU - Kano, Mayuko

AU - Mitsumoto, Kazuki

AU - Kasai, Takatoshi

AU - Kodani, Yu

AU - Nagasaki, Hiroshi

AU - Yamamoto, Naoki

AU - Hagiwara, Daisuke

AU - Goto, Motomitsu

AU - Banno, Ryoichi

AU - Sugimura, Yoshihisa

AU - Arima, Hiroshi

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Arginine-vasopressin (AVP) neurons exist in the hypothalamus, a major region of the diencephalon, and play an essential role in water balance. Here, we established the differentiation method for AVP-secreting neurons from human embryonic stem cells (hESCs) by recapitulating in vitro the in vivo embryonic developmental processes of AVP neurons. At first, the differentiation efficiency was improved. That was achieved through the optimization of the culture condition for obtaining dorsal hypothalamic progenitors. Secondly, the induced AVP neurons were identified by immunohistochemistry and these neurons secreted AVP after potassium chloride stimulation. Additionally, other hypothalamic neuropeptides were also detected, such as oxytocin, corticotropin-releasing hormone, thyrotropin-releasing hormone, pro-opiomelanocortin, agouti-related peptide, orexin, and melanin-concentrating hormone. This is the first report describing the generation of secretory AVP neurons derived from hESCs. This method will be applicable to research using disease models and, potentially, for regenerative medicine of the hypothalamus.

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