Vesicle endocytosis requires dynamin-dependent GTP hydrolysis at a fast CNS synapse

Takayuki Yamashita, Toshihide Hige, Tomoyuki Takahashi

Research output: Contribution to journalArticlepeer-review

127 Citations (Scopus)

Abstract

Molecular dependence of vesicular endocytosis was investigated with capacitance measurements at the calyx of Held terminal in brainstem slices. Intraterminal loading of botulinum toxin E revealed that the rapid capacitance transient implicated as "kiss-and-run" was unrelated to transmitter release. The release-related capacitance change decayed with an endocytotic time constant of 10 to 25 seconds, depending on the magnitude of exocytosis. Presynaptic loading of the nonhydrolyzable guanosine 5′-triphosphate (GTP) analog GTPγS or dynamin-1 proline-rich domain peptide abolished endocytosis. These compounds had no immediate effect on exocytosis, but caused a use-dependent rundown of exocytosis. Thus, the guanosine triphosphatase dynamin-1 is indispensable for vesicle endocytosis at this fast central nervous system (CNS) synapse.

Original languageEnglish
Pages (from-to)124-127
Number of pages4
JournalScience
Volume307
Issue number5706
DOIs
Publication statusPublished - 07-01-2005
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General

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