TY - JOUR
T1 - Virulence characterization of Campylobacter jejuni isolated from resident wild birds in Tokachi area, Japan
AU - Shyaka, Anselme
AU - Kusumoto, Akiko
AU - Chaisowwong, Warangkhana
AU - Okouchi, Yoshiki
AU - Fukumoto, Shinya
AU - Yoshimura, Aya
AU - Kawamoto, Keiko
N1 - Publisher Copyright:
© 2015 The Japanese Society of Veterinary Science.
PY - 2015/8/31
Y1 - 2015/8/31
N2 - The prevalence of Campylobacter jejuni in wild birds is a potential hazard for human and animal health. The aim of this study was to establish the prevalence of C. jejuni in wild birds in Tokachi area, Hokkaido, Japan and investigate their virulence in vitro. In total, 173 cloacal swabs from individual wild birds were collected for the detection of Campylobacter spp. Thirty four samples (19.7%) were positive for Campylobacter of which 94.1% (32/34 samples) were C. jejuni. Additionally, one C. coli and one C. fetus were isolated. Seven C. jejuni isolates (one from crows and the other from pigeons) had important virulence genes including all three CDT genes (cdtA, cdtB and cdtC) and flaA, flaB, ciaB and cadF, and the other isolates were lacking cdtA gene. Further studies on in vitro virulence-associated phenotypes, such as motility assay on soft agar and invasion assay in Caco-2 cells, were performed. The wild bird C. jejuni isolates adhered and invaded human cells. Although the numbers of viable intracellular bacteria of wild bird isolates were lower than a type strain NCTC11168, they persisted at 48-hr and underwent replication in host cells.
AB - The prevalence of Campylobacter jejuni in wild birds is a potential hazard for human and animal health. The aim of this study was to establish the prevalence of C. jejuni in wild birds in Tokachi area, Hokkaido, Japan and investigate their virulence in vitro. In total, 173 cloacal swabs from individual wild birds were collected for the detection of Campylobacter spp. Thirty four samples (19.7%) were positive for Campylobacter of which 94.1% (32/34 samples) were C. jejuni. Additionally, one C. coli and one C. fetus were isolated. Seven C. jejuni isolates (one from crows and the other from pigeons) had important virulence genes including all three CDT genes (cdtA, cdtB and cdtC) and flaA, flaB, ciaB and cadF, and the other isolates were lacking cdtA gene. Further studies on in vitro virulence-associated phenotypes, such as motility assay on soft agar and invasion assay in Caco-2 cells, were performed. The wild bird C. jejuni isolates adhered and invaded human cells. Although the numbers of viable intracellular bacteria of wild bird isolates were lower than a type strain NCTC11168, they persisted at 48-hr and underwent replication in host cells.
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U2 - 10.1292/jvms.15-0090
DO - 10.1292/jvms.15-0090
M3 - Article
C2 - 25843040
AN - SCOPUS:84940702822
SN - 0916-7250
VL - 77
SP - 967
EP - 972
JO - Journal of Veterinary Medical Science
JF - Journal of Veterinary Medical Science
IS - 8
ER -
