Voltage-gated Na+ current availability after step- and spike-shaped conditioning depolarizations of retinal ganglion cells

Soh Hidaka, Andrew T. Ishida

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

We used two conditioning voltage protocols to assess inactivation of voltage-gated Na+ current in retinal ganglion cells. The first protocol tested the possibility, raised by published activation and steady-state inactivation curves, that Na+ ions carry a 'window' current in these cells. The second protocol was used, because these cells spike repetitively in situ, to measure the Na+ current available for activation following spikes. Na+ current activated at test potentials more positive than -65 mV. At test potentials more positive than -55 mV, Na+ current peaked and then declined along a time course that could be fit by the sum of a large, rapidly decaying component, a small, slowly decaying component and a non-decaying component. Both step- and spike-shaped conditioning depolarizations reduced the amount of current available for subsequent activation, sparing the non-decaying 'persistent' component. Most of the Na+ current recovered from this inactivation along a rapid exponential time course (τ=3 ms). The remaining recovery was complete within at least 4 s (at -70 mV). Our use of step depolarizations has identified a current component not anticipated from previous measurements of steady-state inactivation in retinal ganglion cells. Our use of spike-shaped depolarizations shows that Na+ current density at 1 ms after a single spike is roughly 25% of that activated by the conditioning spike, and that recovery from inactivation is 50-90% complete within 10 ms thereafter. Na+ current amplitude declines during spikes repeated at relatively low frequencies, consistent with a slow component of full recovery from inactivation.

Original languageEnglish
Pages (from-to)497-508
Number of pages12
JournalPflugers Archiv European Journal of Physiology
Volume436
Issue number4
DOIs
Publication statusPublished - 1998

All Science Journal Classification (ASJC) codes

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)

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