TY - JOUR
T1 - Voxel-based morphometry of the marmoset brain
T2 - In vivo detection of volume loss in the substantia nigra of the MPTP-treated Parkinson's disease model
AU - Hikishima, K.
AU - Ando, K.
AU - Komaki, Y.
AU - Kawai, K.
AU - Yano, R.
AU - Inoue, T.
AU - Itoh, T.
AU - Yamada, M.
AU - Momoshima, S.
AU - Okano, H. J.
AU - Okano, H.
N1 - Publisher Copyright:
© 2015 IBRO.
PY - 2015/8/6
Y1 - 2015/8/6
N2 - Movement dysfunction in Parkinson's disease (PD) is caused by the degeneration of dopaminergic (DA) neurons in the substantia nigra (SN). Here, we established a method for voxel-based morphometry (VBM) and automatic tissue segmentation of the marmoset monkey brain using a 7-T animal scanner and applied the method to assess DA degeneration in a PD model, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated animals, with tyrosine-hydroxylase staining. The most significant decreases of local tissue volume were detected in the bilateral SN of MPTP-treated marmoset brains (-53.0% in right and -46.5% in left) and corresponded with the location of DA neurodegeneration found in histology (-65.4% in right). In addition to the SN, the decreases were also confirmed in the locus coeruleus, and lateral hypothalamus. VBM using 7-T MRI was effective in detecting volume loss in the SN of the PD-model marmoset. This study provides a potential basis for the application of VBM with ultra-high field MRI in the clinical diagnosis of PD. The developed method may also offer value in automatic whole-brain evaluation of structural changes for the marmoset monkey.
AB - Movement dysfunction in Parkinson's disease (PD) is caused by the degeneration of dopaminergic (DA) neurons in the substantia nigra (SN). Here, we established a method for voxel-based morphometry (VBM) and automatic tissue segmentation of the marmoset monkey brain using a 7-T animal scanner and applied the method to assess DA degeneration in a PD model, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated animals, with tyrosine-hydroxylase staining. The most significant decreases of local tissue volume were detected in the bilateral SN of MPTP-treated marmoset brains (-53.0% in right and -46.5% in left) and corresponded with the location of DA neurodegeneration found in histology (-65.4% in right). In addition to the SN, the decreases were also confirmed in the locus coeruleus, and lateral hypothalamus. VBM using 7-T MRI was effective in detecting volume loss in the SN of the PD-model marmoset. This study provides a potential basis for the application of VBM with ultra-high field MRI in the clinical diagnosis of PD. The developed method may also offer value in automatic whole-brain evaluation of structural changes for the marmoset monkey.
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U2 - 10.1016/j.neuroscience.2015.05.041
DO - 10.1016/j.neuroscience.2015.05.041
M3 - Article
C2 - 26012491
AN - SCOPUS:84931271760
SN - 0306-4522
VL - 300
SP - 585
EP - 592
JO - Neuroscience
JF - Neuroscience
ER -