WARTS tumor suppressor is phosphorylated by Cdc2/cyclin B at spindle poles during mitosis

Tetsuro Morisaki; Toru Hirota; Shin ichi Iida; Tomotoshi Marumoto; Toshihiro Hara; Yasuyuki Nishiyama; Michio Kawasuzi; Takehisa Hiraoka; Tatsuyuki Mimori; Norie Araki; Ichiro Izawa; Masaki Inagaki; Hideyuki Saya

doi:10.1016/S0014-5793(02)03360-4

WARTS tumor suppressor is phosphorylated by Cdc2/cyclin B at spindle poles during mitosis

Tetsuro Morisaki, Toru Hirota, Shin ichi Iida, Tomotoshi Marumoto, Toshihiro Hara, Yasuyuki Nishiyama, Michio Kawasuzi, Takehisa Hiraoka, Tatsuyuki Mimori, Norie Araki, Ichiro Izawa, Masaki Inagaki, Hideyuki Saya

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38 Citations (Scopus)

Abstract

Identification of physiological substrates for Cdc2/cyclin B is crucial for understanding the functional link between mitotic events and Cdc2/cyclin B activation. A human homologue of the Drosophila warts tumor suppressor, termed WARTS, is a serine/threonine kinase and a dynamic component of the mitotic apparatus. We have found that Cdc2/cyclin B forms a complex with a fraction of WARTS in the centrosome and phosphorylates the Ser613 site of WARTS during mitosis. Immunocytochemical analysis has shown that the S613-phosphorylated WARTS appears in the spindle poles at prometaphase and disappears at telophase. Our findings suggest that Cdc/cyclin B regulates functions of WARTS on the mitotic apparatus.

Original language	English
Pages (from-to)	319-324
Number of pages	6
Journal	FEBS Letters
Volume	529
Issue number	2-3
DOIs	https://doi.org/10.1016/S0014-5793(02)03360-4
Publication status	Published - 09-10-2002
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(02)03360-4

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title = "WARTS tumor suppressor is phosphorylated by Cdc2/cyclin B at spindle poles during mitosis",

abstract = "Identification of physiological substrates for Cdc2/cyclin B is crucial for understanding the functional link between mitotic events and Cdc2/cyclin B activation. A human homologue of the Drosophila warts tumor suppressor, termed WARTS, is a serine/threonine kinase and a dynamic component of the mitotic apparatus. We have found that Cdc2/cyclin B forms a complex with a fraction of WARTS in the centrosome and phosphorylates the Ser613 site of WARTS during mitosis. Immunocytochemical analysis has shown that the S613-phosphorylated WARTS appears in the spindle poles at prometaphase and disappears at telophase. Our findings suggest that Cdc/cyclin B regulates functions of WARTS on the mitotic apparatus.",

author = "Tetsuro Morisaki and Toru Hirota and Iida, {Shin ichi} and Tomotoshi Marumoto and Toshihiro Hara and Yasuyuki Nishiyama and Michio Kawasuzi and Takehisa Hiraoka and Tatsuyuki Mimori and Norie Araki and Ichiro Izawa and Masaki Inagaki and Hideyuki Saya",

note = "Funding Information: We thank Dr. H. Goto for valuable suggestions; Dr. J. Moon for editing the manuscript; and Y. Fukushima for secretarial assistance. We are also grateful to members of the Gene Technology Center in Kumamoto University for their important contributions to the experiments. This work was supported by a grant for Cancer Research from the Japanese Ministry of Education, Science, Sports and Culture (H.S.), and the {\textquoteleft}Research for the Future{\textquoteright} program (H.S.) of the Japan Society for the Promotion of Science.",

year = "2002",

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doi = "10.1016/S0014-5793(02)03360-4",

language = "English",

volume = "529",

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journal = "FEBS Letters",

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TY - JOUR

T1 - WARTS tumor suppressor is phosphorylated by Cdc2/cyclin B at spindle poles during mitosis

AU - Morisaki, Tetsuro

AU - Hirota, Toru

AU - Iida, Shin ichi

AU - Marumoto, Tomotoshi

AU - Hara, Toshihiro

AU - Nishiyama, Yasuyuki

AU - Kawasuzi, Michio

AU - Hiraoka, Takehisa

AU - Mimori, Tatsuyuki

AU - Araki, Norie

AU - Izawa, Ichiro

AU - Inagaki, Masaki

AU - Saya, Hideyuki

N1 - Funding Information: We thank Dr. H. Goto for valuable suggestions; Dr. J. Moon for editing the manuscript; and Y. Fukushima for secretarial assistance. We are also grateful to members of the Gene Technology Center in Kumamoto University for their important contributions to the experiments. This work was supported by a grant for Cancer Research from the Japanese Ministry of Education, Science, Sports and Culture (H.S.), and the ‘Research for the Future’ program (H.S.) of the Japan Society for the Promotion of Science.

PY - 2002/10/9

Y1 - 2002/10/9

N2 - Identification of physiological substrates for Cdc2/cyclin B is crucial for understanding the functional link between mitotic events and Cdc2/cyclin B activation. A human homologue of the Drosophila warts tumor suppressor, termed WARTS, is a serine/threonine kinase and a dynamic component of the mitotic apparatus. We have found that Cdc2/cyclin B forms a complex with a fraction of WARTS in the centrosome and phosphorylates the Ser613 site of WARTS during mitosis. Immunocytochemical analysis has shown that the S613-phosphorylated WARTS appears in the spindle poles at prometaphase and disappears at telophase. Our findings suggest that Cdc/cyclin B regulates functions of WARTS on the mitotic apparatus.

AB - Identification of physiological substrates for Cdc2/cyclin B is crucial for understanding the functional link between mitotic events and Cdc2/cyclin B activation. A human homologue of the Drosophila warts tumor suppressor, termed WARTS, is a serine/threonine kinase and a dynamic component of the mitotic apparatus. We have found that Cdc2/cyclin B forms a complex with a fraction of WARTS in the centrosome and phosphorylates the Ser613 site of WARTS during mitosis. Immunocytochemical analysis has shown that the S613-phosphorylated WARTS appears in the spindle poles at prometaphase and disappears at telophase. Our findings suggest that Cdc/cyclin B regulates functions of WARTS on the mitotic apparatus.

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ER -

WARTS tumor suppressor is phosphorylated by Cdc2/cyclin B at spindle poles during mitosis

Abstract

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