Whole genomic analysis of a porcine-like human G5P[6] rotavirus strain isolated from a child with diarrhoea and encephalopathy in Japan

Satoshi Komoto, Yoshimasa Maeno, Mayuko Tomita, Tsuyoshi Matsuoka, Masaharu Ohfu, Toshifumi Yodoshi, Hideki Akeda, Koki Taniguchi

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

An unusual rotavirus strain, Ryukyu-1120, with G5P[6] genotypes (RVA/Human-wt/JPN/Ryukyu- 1120/2011/G5P[6]) was identified in a stool specimen from a hospitalized child aged 4 years who showed diarrhoea and encephalopathy. In this study, we sequenced and characterized the complete genome of strain Ryukyu-1120. On whole genomic analysis, this strain was found to have a unique genotype constellation: G5-P[6]-I5-R1-C1-M1-A8-N1-T1-E1-H1. The VP6 and NSP1 genotypes I5 and A8 are those commonly found in porcine strains. Furthermore, phylogenetic analysis indicated that each of the 11 genes of strain Ryukyu-1120 appeared to be of porcine origin. Thus, strain Ryukyu-1120 was found to have a porcine rotavirus genetic backbone and is likely to be of porcine origin. To our knowledge, this is the first report of wholegenome- based characterization of the emerging G5P[6] strains in Asian countries. Our observations will provide important insights into the origin of G5P[6] strains and the dynamic interactions between human and porcine rotavirus strains.

Original languageEnglish
Pages (from-to)1568-1575
Number of pages8
JournalJournal of General Virology
Volume94
Issue numberPART7
DOIs
Publication statusPublished - 01-07-2013

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Rotavirus
Brain Diseases
Diarrhea
Japan
Swine
Genotype
Hospitalized Child
Genome
Genes

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

Komoto, Satoshi ; Maeno, Yoshimasa ; Tomita, Mayuko ; Matsuoka, Tsuyoshi ; Ohfu, Masaharu ; Yodoshi, Toshifumi ; Akeda, Hideki ; Taniguchi, Koki. / Whole genomic analysis of a porcine-like human G5P[6] rotavirus strain isolated from a child with diarrhoea and encephalopathy in Japan. In: Journal of General Virology. 2013 ; Vol. 94, No. PART7. pp. 1568-1575.
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Whole genomic analysis of a porcine-like human G5P[6] rotavirus strain isolated from a child with diarrhoea and encephalopathy in Japan. / Komoto, Satoshi; Maeno, Yoshimasa; Tomita, Mayuko; Matsuoka, Tsuyoshi; Ohfu, Masaharu; Yodoshi, Toshifumi; Akeda, Hideki; Taniguchi, Koki.

In: Journal of General Virology, Vol. 94, No. PART7, 01.07.2013, p. 1568-1575.

Research output: Contribution to journalArticle

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