Whole genomic analysis of human G12P[6] and G12P[8] rotavirus strains that have emerged in Myanmar

Tomihiko Ide, Satoshi Komoto, Kyoko Moriguchi, Khaing Win Htun, Yi Yi Myint, Theingi Win Myat, Kyaw Zin Thant, Hlaing Myat Thu, Mo Mo Win, Htun Naing Oo, Than Htut, Mitsutaka Wakuda, Francis Ekow Dennis, Kei Haga, Yoshiki Fujii, Kazuhiko Katayama, Shofiqur Rahman, Sa Van Nguyen, Kouji Umeda, Keiji OgumaTakao Tsuji, Koki Taniguchi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

G12 rotaviruses are emerging rotavirus strains causing severe diarrhea in infants and young children worldwide. However, the whole genomes of only a few G12 strains have been fully sequenced and analyzed. In this study, we sequenced and characterized the complete genomes of six G12 strains (RVA/Human-tc/MMR/A14/2011/G12P[8], RVA/Human-tc/MMR/A23/2011/G12P[6], RVA/Human-tc/MMR/A25/2011/G12P[8], RVA/Human-tc/MMR/P02/2011/G12P[8], RVA/Human-tc/MMR/P39/2011/G12P[8], and RVA/Human-tc/MMR/P43/2011/G12P[8]) detected in six stool samples from children with acute gastroenteritis in Myanmar. On whole genomic analysis, all six Myanmarese G12 strains were found to have a Wa-like genetic backbone: G12-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1 for strains A14, A25, P02, P39, and P43, and G12-P[6]-I1-R1-C1-M1-A1-N1-T1-E1-H1 for strain A23. Phylogenetic analysis showed that most genes of the six strains examined in this study were genetically related to globally circulating human G1, G3, G9, and G12 strains. Of note is that the NSP4 gene of strain A23 exhibited the closest relationship with the cognate genes of human-like bovine strains as well as human strains, suggesting the occurrence of reassortment between human and bovine strains. Furthermore, strains A14, A25, P02, P39, and P43 were very closely related to one another in all the 11 gene segments, indicating derivation of the five strains from a common origin. On the other hand, strain A23 consistently formed distinct clusters as to all the 11 gene segments, indicating a distinct origin of strain A23 from that of strains A14, A25, P02, P39, and P43. To our knowledge, this is the first report on whole genome-based characterization of G12 strains that have emerged in Myanmar. Our observations will provide important insights into the evolutionary dynamics of spreading G12 rotaviruses in Asia.

Original languageEnglish
Article numbere0124965
JournalPloS one
Volume10
Issue number5
DOIs
Publication statusPublished - 04-05-2015

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Myanmar
Rotavirus
genomics
Genes
Genome
Gastroenteritis
genes
genome
Diarrhea

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Ide, Tomihiko ; Komoto, Satoshi ; Moriguchi, Kyoko ; Htun, Khaing Win ; Myint, Yi Yi ; Myat, Theingi Win ; Thant, Kyaw Zin ; Thu, Hlaing Myat ; Win, Mo Mo ; Oo, Htun Naing ; Htut, Than ; Wakuda, Mitsutaka ; Dennis, Francis Ekow ; Haga, Kei ; Fujii, Yoshiki ; Katayama, Kazuhiko ; Rahman, Shofiqur ; Van Nguyen, Sa ; Umeda, Kouji ; Oguma, Keiji ; Tsuji, Takao ; Taniguchi, Koki. / Whole genomic analysis of human G12P[6] and G12P[8] rotavirus strains that have emerged in Myanmar. In: PloS one. 2015 ; Vol. 10, No. 5.
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title = "Whole genomic analysis of human G12P[6] and G12P[8] rotavirus strains that have emerged in Myanmar",
abstract = "G12 rotaviruses are emerging rotavirus strains causing severe diarrhea in infants and young children worldwide. However, the whole genomes of only a few G12 strains have been fully sequenced and analyzed. In this study, we sequenced and characterized the complete genomes of six G12 strains (RVA/Human-tc/MMR/A14/2011/G12P[8], RVA/Human-tc/MMR/A23/2011/G12P[6], RVA/Human-tc/MMR/A25/2011/G12P[8], RVA/Human-tc/MMR/P02/2011/G12P[8], RVA/Human-tc/MMR/P39/2011/G12P[8], and RVA/Human-tc/MMR/P43/2011/G12P[8]) detected in six stool samples from children with acute gastroenteritis in Myanmar. On whole genomic analysis, all six Myanmarese G12 strains were found to have a Wa-like genetic backbone: G12-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1 for strains A14, A25, P02, P39, and P43, and G12-P[6]-I1-R1-C1-M1-A1-N1-T1-E1-H1 for strain A23. Phylogenetic analysis showed that most genes of the six strains examined in this study were genetically related to globally circulating human G1, G3, G9, and G12 strains. Of note is that the NSP4 gene of strain A23 exhibited the closest relationship with the cognate genes of human-like bovine strains as well as human strains, suggesting the occurrence of reassortment between human and bovine strains. Furthermore, strains A14, A25, P02, P39, and P43 were very closely related to one another in all the 11 gene segments, indicating derivation of the five strains from a common origin. On the other hand, strain A23 consistently formed distinct clusters as to all the 11 gene segments, indicating a distinct origin of strain A23 from that of strains A14, A25, P02, P39, and P43. To our knowledge, this is the first report on whole genome-based characterization of G12 strains that have emerged in Myanmar. Our observations will provide important insights into the evolutionary dynamics of spreading G12 rotaviruses in Asia.",
author = "Tomihiko Ide and Satoshi Komoto and Kyoko Moriguchi and Htun, {Khaing Win} and Myint, {Yi Yi} and Myat, {Theingi Win} and Thant, {Kyaw Zin} and Thu, {Hlaing Myat} and Win, {Mo Mo} and Oo, {Htun Naing} and Than Htut and Mitsutaka Wakuda and Dennis, {Francis Ekow} and Kei Haga and Yoshiki Fujii and Kazuhiko Katayama and Shofiqur Rahman and {Van Nguyen}, Sa and Kouji Umeda and Keiji Oguma and Takao Tsuji and Koki Taniguchi",
year = "2015",
month = "5",
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Ide, T, Komoto, S, Moriguchi, K, Htun, KW, Myint, YY, Myat, TW, Thant, KZ, Thu, HM, Win, MM, Oo, HN, Htut, T, Wakuda, M, Dennis, FE, Haga, K, Fujii, Y, Katayama, K, Rahman, S, Van Nguyen, S, Umeda, K, Oguma, K, Tsuji, T & Taniguchi, K 2015, 'Whole genomic analysis of human G12P[6] and G12P[8] rotavirus strains that have emerged in Myanmar', PloS one, vol. 10, no. 5, e0124965. https://doi.org/10.1371/journal.pone.0124965

Whole genomic analysis of human G12P[6] and G12P[8] rotavirus strains that have emerged in Myanmar. / Ide, Tomihiko; Komoto, Satoshi; Moriguchi, Kyoko; Htun, Khaing Win; Myint, Yi Yi; Myat, Theingi Win; Thant, Kyaw Zin; Thu, Hlaing Myat; Win, Mo Mo; Oo, Htun Naing; Htut, Than; Wakuda, Mitsutaka; Dennis, Francis Ekow; Haga, Kei; Fujii, Yoshiki; Katayama, Kazuhiko; Rahman, Shofiqur; Van Nguyen, Sa; Umeda, Kouji; Oguma, Keiji; Tsuji, Takao; Taniguchi, Koki.

In: PloS one, Vol. 10, No. 5, e0124965, 04.05.2015.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Whole genomic analysis of human G12P[6] and G12P[8] rotavirus strains that have emerged in Myanmar

AU - Ide, Tomihiko

AU - Komoto, Satoshi

AU - Moriguchi, Kyoko

AU - Htun, Khaing Win

AU - Myint, Yi Yi

AU - Myat, Theingi Win

AU - Thant, Kyaw Zin

AU - Thu, Hlaing Myat

AU - Win, Mo Mo

AU - Oo, Htun Naing

AU - Htut, Than

AU - Wakuda, Mitsutaka

AU - Dennis, Francis Ekow

AU - Haga, Kei

AU - Fujii, Yoshiki

AU - Katayama, Kazuhiko

AU - Rahman, Shofiqur

AU - Van Nguyen, Sa

AU - Umeda, Kouji

AU - Oguma, Keiji

AU - Tsuji, Takao

AU - Taniguchi, Koki

PY - 2015/5/4

Y1 - 2015/5/4

N2 - G12 rotaviruses are emerging rotavirus strains causing severe diarrhea in infants and young children worldwide. However, the whole genomes of only a few G12 strains have been fully sequenced and analyzed. In this study, we sequenced and characterized the complete genomes of six G12 strains (RVA/Human-tc/MMR/A14/2011/G12P[8], RVA/Human-tc/MMR/A23/2011/G12P[6], RVA/Human-tc/MMR/A25/2011/G12P[8], RVA/Human-tc/MMR/P02/2011/G12P[8], RVA/Human-tc/MMR/P39/2011/G12P[8], and RVA/Human-tc/MMR/P43/2011/G12P[8]) detected in six stool samples from children with acute gastroenteritis in Myanmar. On whole genomic analysis, all six Myanmarese G12 strains were found to have a Wa-like genetic backbone: G12-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1 for strains A14, A25, P02, P39, and P43, and G12-P[6]-I1-R1-C1-M1-A1-N1-T1-E1-H1 for strain A23. Phylogenetic analysis showed that most genes of the six strains examined in this study were genetically related to globally circulating human G1, G3, G9, and G12 strains. Of note is that the NSP4 gene of strain A23 exhibited the closest relationship with the cognate genes of human-like bovine strains as well as human strains, suggesting the occurrence of reassortment between human and bovine strains. Furthermore, strains A14, A25, P02, P39, and P43 were very closely related to one another in all the 11 gene segments, indicating derivation of the five strains from a common origin. On the other hand, strain A23 consistently formed distinct clusters as to all the 11 gene segments, indicating a distinct origin of strain A23 from that of strains A14, A25, P02, P39, and P43. To our knowledge, this is the first report on whole genome-based characterization of G12 strains that have emerged in Myanmar. Our observations will provide important insights into the evolutionary dynamics of spreading G12 rotaviruses in Asia.

AB - G12 rotaviruses are emerging rotavirus strains causing severe diarrhea in infants and young children worldwide. However, the whole genomes of only a few G12 strains have been fully sequenced and analyzed. In this study, we sequenced and characterized the complete genomes of six G12 strains (RVA/Human-tc/MMR/A14/2011/G12P[8], RVA/Human-tc/MMR/A23/2011/G12P[6], RVA/Human-tc/MMR/A25/2011/G12P[8], RVA/Human-tc/MMR/P02/2011/G12P[8], RVA/Human-tc/MMR/P39/2011/G12P[8], and RVA/Human-tc/MMR/P43/2011/G12P[8]) detected in six stool samples from children with acute gastroenteritis in Myanmar. On whole genomic analysis, all six Myanmarese G12 strains were found to have a Wa-like genetic backbone: G12-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1 for strains A14, A25, P02, P39, and P43, and G12-P[6]-I1-R1-C1-M1-A1-N1-T1-E1-H1 for strain A23. Phylogenetic analysis showed that most genes of the six strains examined in this study were genetically related to globally circulating human G1, G3, G9, and G12 strains. Of note is that the NSP4 gene of strain A23 exhibited the closest relationship with the cognate genes of human-like bovine strains as well as human strains, suggesting the occurrence of reassortment between human and bovine strains. Furthermore, strains A14, A25, P02, P39, and P43 were very closely related to one another in all the 11 gene segments, indicating derivation of the five strains from a common origin. On the other hand, strain A23 consistently formed distinct clusters as to all the 11 gene segments, indicating a distinct origin of strain A23 from that of strains A14, A25, P02, P39, and P43. To our knowledge, this is the first report on whole genome-based characterization of G12 strains that have emerged in Myanmar. Our observations will provide important insights into the evolutionary dynamics of spreading G12 rotaviruses in Asia.

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