Y-chromosomal short tandem repeats haplotyping from vaginal swabs using a chelating resin-based DNA extraction method and a dual-round polymerase chain reaction

Mineo Iwasa, Hiroyoshi Koyama, Tsukasa Tsuchimochi, Yoshitaka Maeno, Ichiro Isobe, Yoshimi Seko-Nakamura, Jun Monma-Ohtaki, Tomohiro Matsumoto, Masataka Nagao

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Reported are 2 autopsy cases in which Y-chromosomal microsatellite short tandem repeats DYS19, DYS389I and II, DYS390, and DYS393 could be haplotyped with vaginal swabs by using a Chelex 100-based DNA extraction method and dual-round polymerase chain reaction. The extraction of DNA from vaginal swabs by using this method was as efficient or more efficient than using proteinase K and phenol-chloroform extraction or the alkaline lysis methods. Y-chromosomal microsatellite short tandem repeats haplotyping based on the dual-round polymerase chain reaction method provided genotypes from all the loci determined. Although amplification of Y-chromosomal microsatellite short tandem repeats loci is not directly involved in the existence of spermatozoa, it is considerably advantageous for male individualization from body fluid mixture stains in criminal cases.

Original languageEnglish
Pages (from-to)303-305
Number of pages3
JournalAmerican Journal of Forensic Medicine and Pathology
Volume24
Issue number3
DOIs
Publication statusPublished - 09-2003
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

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