TY - JOUR
T1 - Y-chromosomal short tandem repeats haplotyping from vaginal swabs using a chelating resin-based DNA extraction method and a dual-round polymerase chain reaction
AU - Iwasa, Mineo
AU - Koyama, Hiroyoshi
AU - Tsuchimochi, Tsukasa
AU - Maeno, Yoshitaka
AU - Isobe, Ichiro
AU - Seko-Nakamura, Yoshimi
AU - Monma-Ohtaki, Jun
AU - Matsumoto, Tomohiro
AU - Nagao, Masataka
PY - 2003/9
Y1 - 2003/9
N2 - Reported are 2 autopsy cases in which Y-chromosomal microsatellite short tandem repeats DYS19, DYS389I and II, DYS390, and DYS393 could be haplotyped with vaginal swabs by using a Chelex 100-based DNA extraction method and dual-round polymerase chain reaction. The extraction of DNA from vaginal swabs by using this method was as efficient or more efficient than using proteinase K and phenol-chloroform extraction or the alkaline lysis methods. Y-chromosomal microsatellite short tandem repeats haplotyping based on the dual-round polymerase chain reaction method provided genotypes from all the loci determined. Although amplification of Y-chromosomal microsatellite short tandem repeats loci is not directly involved in the existence of spermatozoa, it is considerably advantageous for male individualization from body fluid mixture stains in criminal cases.
AB - Reported are 2 autopsy cases in which Y-chromosomal microsatellite short tandem repeats DYS19, DYS389I and II, DYS390, and DYS393 could be haplotyped with vaginal swabs by using a Chelex 100-based DNA extraction method and dual-round polymerase chain reaction. The extraction of DNA from vaginal swabs by using this method was as efficient or more efficient than using proteinase K and phenol-chloroform extraction or the alkaline lysis methods. Y-chromosomal microsatellite short tandem repeats haplotyping based on the dual-round polymerase chain reaction method provided genotypes from all the loci determined. Although amplification of Y-chromosomal microsatellite short tandem repeats loci is not directly involved in the existence of spermatozoa, it is considerably advantageous for male individualization from body fluid mixture stains in criminal cases.
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U2 - 10.1097/01.paf.0000083452.84512.36
DO - 10.1097/01.paf.0000083452.84512.36
M3 - Article
C2 - 12960670
AN - SCOPUS:0042819685
SN - 0195-7910
VL - 24
SP - 303
EP - 305
JO - American Journal of Forensic Medicine and Pathology
JF - American Journal of Forensic Medicine and Pathology
IS - 3
ER -
