Zebrafish Dmrta2 regulates neurogenesis in the telencephalon

Akio Yoshizawa, Yoshinari Nakahara, Toshiaki Izawa, Tohru Ishitani, Makiko Tsutsumi, Atsushi Kuroiwa, Motoyuki Itoh, Yutaka Kikuchi

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Although recent findings showed that some Drosophila doublesex and Caenorhabditis elegans mab-3 related genes are expressed in neural tissues during development, their functions have not been fully elucidated. Here, we isolated a zebrafish mutant, ha2, that shows defects in telencephalic neurogenesis and found that ha2 encodes Doublesex and MAB-3 related transcription factor like family A2 (Dmrta2). dmrta2 expression is restricted to the telencephalon, diencephalon and olfactory placode during somitogenesis. We found that the expression of the proneural gene, neurogenin1, in the posterior and dorsal region of telencephalon (posterior-dorsal telencephalon) is markedly reduced in this mutant at the 14-somite stage without any defects in cell proliferation or cell death. In contrast, the telencephalic expression of her6, a Hes-related gene that is known to encode a negative regulator of neurogenin1, expands dramatically in the ha2 mutant. Based on over-expression experiments and epistatic analyses, we propose that zebrafish Dmrta2 controls neurogenin1 expression by repressing her6 in the posterior-dorsal telencephalon. Furthermore, the expression domains of the telencephalic marker genes, foxg1 and emx3, and the neuronal differentiation gene, neurod, are downregulated in the ha2 posterior-dorsal telencephalon during somitogenesis. These results suggest that Dmrta2 plays important roles in the specification of the posterior-dorsal telencephalic cell fate during somitogenesis.

Original languageEnglish
Pages (from-to)1097-1109
Number of pages13
JournalGenes to Cells
Volume16
Issue number11
DOIs
Publication statusPublished - 01-11-2011

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Telencephalon
Neurogenesis
Zebrafish
Genes
Transcription Factor 3
Diencephalon
Somites
Caenorhabditis elegans
varespladib methyl
Drosophila
Cell Death
Down-Regulation
Cell Proliferation
Gene Expression

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

Cite this

Yoshizawa, A., Nakahara, Y., Izawa, T., Ishitani, T., Tsutsumi, M., Kuroiwa, A., ... Kikuchi, Y. (2011). Zebrafish Dmrta2 regulates neurogenesis in the telencephalon. Genes to Cells, 16(11), 1097-1109. https://doi.org/10.1111/j.1365-2443.2011.01555.x
Yoshizawa, Akio ; Nakahara, Yoshinari ; Izawa, Toshiaki ; Ishitani, Tohru ; Tsutsumi, Makiko ; Kuroiwa, Atsushi ; Itoh, Motoyuki ; Kikuchi, Yutaka. / Zebrafish Dmrta2 regulates neurogenesis in the telencephalon. In: Genes to Cells. 2011 ; Vol. 16, No. 11. pp. 1097-1109.
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Yoshizawa, A, Nakahara, Y, Izawa, T, Ishitani, T, Tsutsumi, M, Kuroiwa, A, Itoh, M & Kikuchi, Y 2011, 'Zebrafish Dmrta2 regulates neurogenesis in the telencephalon', Genes to Cells, vol. 16, no. 11, pp. 1097-1109. https://doi.org/10.1111/j.1365-2443.2011.01555.x

Zebrafish Dmrta2 regulates neurogenesis in the telencephalon. / Yoshizawa, Akio; Nakahara, Yoshinari; Izawa, Toshiaki; Ishitani, Tohru; Tsutsumi, Makiko; Kuroiwa, Atsushi; Itoh, Motoyuki; Kikuchi, Yutaka.

In: Genes to Cells, Vol. 16, No. 11, 01.11.2011, p. 1097-1109.

Research output: Contribution to journalArticle

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Yoshizawa A, Nakahara Y, Izawa T, Ishitani T, Tsutsumi M, Kuroiwa A et al. Zebrafish Dmrta2 regulates neurogenesis in the telencephalon. Genes to Cells. 2011 Nov 1;16(11):1097-1109. https://doi.org/10.1111/j.1365-2443.2011.01555.x