Zinc and magnesium ions synergistically inhibit superoxide generation by cultured human neutrophils-a promising candidate formulation for amnioinfusion fluid

Toshiyuki Uchida, Hiroaki Itoh, Yuki Nakamura, Yukiko Kobayashi, Kyuya Hirai, Kazunao Suzuki, Kazuhiro Sugihara, Naohiro Kanayama, Mitsuo Hiramatsu

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Oligohydramnios is often caused by the premature rupturing of membranes and subsequent intrauterine infections, such as chorioamnionitis, in which event oxidative stress is hypothesized to be closely associated with the damage to the fetal organs. The clinical efficiency of amnioinfusion using warmed saline in cases of premature rupture of membranes is still controversial, especially concerning the prognosis for the fetus. In the present study, we found that human amniotic fluid . per se suppresses the release of superoxide from cultured human neutrophils, suggesting an acute or chronic shortage of amniotic fluid in cases of premature rupture of membranes can affect the shielding of intrauterine organs from oxidative stress. The aim of this study was to propose a formula of zinc and magnesium ions in saline for amnioinfusion, by assessing antioxidative activities. A combination of 5μM zinc and 5. mM magnesium in saline synergistically inhibited superoxide production by cultured human neutrophils, equivalent to human amniotic fluid. The intraperitoneal administration of this formula significantly improved the survival rate in a rat model of peritonitis compared to the saline control (46.7% vs. 10%). The combination of these metals with saline may thus be a promising formula for an amnioinfusion fluid with the capacity to protect fetal organs from oxidative stress.

Original languageEnglish
Pages (from-to)209-213
Number of pages5
JournalJournal of Reproductive Immunology
Volume85
Issue number2
DOIs
Publication statusPublished - 06-2010
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Reproductive Medicine
  • Obstetrics and Gynaecology

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