ZNF10 inhibits HIV-1 LTR activity through interaction with NF-κB and Sp1 binding motifs

Hironori Nishitsuji, Leila Sawada, Ryuichi Sugiyama, Hiroshi Takaku

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Abstract Kruppel-associated box-containing zinc finger (KRAB-ZNF) genes constitute the single largest gene family of transcriptional repressors in the genomes of higher organisms. In this study, we isolated 52 cDNA clones of KRAB-ZFPs from U1 cell lines and screened them to identify which were capable of regulating HIV-1 gene expression. We identified 5 KRAB-ZFPs that suppressed ≥50% of HIV-1 LTR. Of the 5 identified KRAB-ZFPs, the expression of ZNF10 significantly enhanced the transcriptional repression activity of the LTR compared with other ZNFs. In addition, the depletion of endogenous ZNF10 led to the activation of HIV-1 LTR. The repressor activity of ZNF10 was required for TRIM28, SETDB1 and HP1-gamma binding. These results indicate that ZNF10 could be involved in a potent intrinsic antiretroviral defense.

Original languageEnglish
Article number37225
Pages (from-to)2019-2025
Number of pages7
JournalFEBS Letters
Volume589
Issue number15
DOIs
Publication statusPublished - 03-07-2015
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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