TY - JOUR
T1 - ZNF10 inhibits HIV-1 LTR activity through interaction with NF-κB and Sp1 binding motifs
AU - Nishitsuji, Hironori
AU - Sawada, Leila
AU - Sugiyama, Ryuichi
AU - Takaku, Hiroshi
N1 - Funding Information:
We thank Dr. M. Abe, and Mr. H. Sato for their technical assistance. This work was supported in part by a Grant-in-Aid for Science Research (C) from the Japan Society for the Promotion of Science (JSPS), Japan; by a Grant-in-Aid for AIDS research from the Ministry of Health, Labor, and Welfare, Japan ; and by a grant from the Strategic Research Foundation Grant-aided Project for Private Universities from the Ministry of Education, Culture, Sport, Science, and Technology, Japan (MEXT).
Publisher Copyright:
© 2015 Published by Elsevier B.V.
PY - 2015/7/3
Y1 - 2015/7/3
N2 - Abstract Kruppel-associated box-containing zinc finger (KRAB-ZNF) genes constitute the single largest gene family of transcriptional repressors in the genomes of higher organisms. In this study, we isolated 52 cDNA clones of KRAB-ZFPs from U1 cell lines and screened them to identify which were capable of regulating HIV-1 gene expression. We identified 5 KRAB-ZFPs that suppressed ≥50% of HIV-1 LTR. Of the 5 identified KRAB-ZFPs, the expression of ZNF10 significantly enhanced the transcriptional repression activity of the LTR compared with other ZNFs. In addition, the depletion of endogenous ZNF10 led to the activation of HIV-1 LTR. The repressor activity of ZNF10 was required for TRIM28, SETDB1 and HP1-gamma binding. These results indicate that ZNF10 could be involved in a potent intrinsic antiretroviral defense.
AB - Abstract Kruppel-associated box-containing zinc finger (KRAB-ZNF) genes constitute the single largest gene family of transcriptional repressors in the genomes of higher organisms. In this study, we isolated 52 cDNA clones of KRAB-ZFPs from U1 cell lines and screened them to identify which were capable of regulating HIV-1 gene expression. We identified 5 KRAB-ZFPs that suppressed ≥50% of HIV-1 LTR. Of the 5 identified KRAB-ZFPs, the expression of ZNF10 significantly enhanced the transcriptional repression activity of the LTR compared with other ZNFs. In addition, the depletion of endogenous ZNF10 led to the activation of HIV-1 LTR. The repressor activity of ZNF10 was required for TRIM28, SETDB1 and HP1-gamma binding. These results indicate that ZNF10 could be involved in a potent intrinsic antiretroviral defense.
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U2 - 10.1016/j.febslet.2015.06.013
DO - 10.1016/j.febslet.2015.06.013
M3 - Article
C2 - 26096782
AN - SCOPUS:84934443560
SN - 0014-5793
VL - 589
SP - 2019
EP - 2025
JO - FEBS Letters
JF - FEBS Letters
IS - 15
M1 - 37225
ER -