ZNF10 inhibits HIV-1 LTR activity through interaction with NF-κB and Sp1 binding motifs

Hironori Nishitsuji; Leila Sawada; Ryuichi Sugiyama; Hiroshi Takaku

doi:10.1016/j.febslet.2015.06.013

ZNF10 inhibits HIV-1 LTR activity through interaction with NF-κB and Sp1 binding motifs

Hironori Nishitsuji, Leila Sawada, Ryuichi Sugiyama, Hiroshi Takaku

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

Abstract

Abstract Kruppel-associated box-containing zinc finger (KRAB-ZNF) genes constitute the single largest gene family of transcriptional repressors in the genomes of higher organisms. In this study, we isolated 52 cDNA clones of KRAB-ZFPs from U1 cell lines and screened them to identify which were capable of regulating HIV-1 gene expression. We identified 5 KRAB-ZFPs that suppressed ≥50% of HIV-1 LTR. Of the 5 identified KRAB-ZFPs, the expression of ZNF10 significantly enhanced the transcriptional repression activity of the LTR compared with other ZNFs. In addition, the depletion of endogenous ZNF10 led to the activation of HIV-1 LTR. The repressor activity of ZNF10 was required for TRIM28, SETDB1 and HP1-gamma binding. These results indicate that ZNF10 could be involved in a potent intrinsic antiretroviral defense.

Original language	English
Article number	37225
Pages (from-to)	2019-2025
Number of pages	7
Journal	FEBS Letters
Volume	589
Issue number	15
DOIs	https://doi.org/10.1016/j.febslet.2015.06.013
Publication status	Published - 03-07-2015
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.febslet.2015.06.013

Cite this

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title = "ZNF10 inhibits HIV-1 LTR activity through interaction with NF-κB and Sp1 binding motifs",

abstract = "Abstract Kruppel-associated box-containing zinc finger (KRAB-ZNF) genes constitute the single largest gene family of transcriptional repressors in the genomes of higher organisms. In this study, we isolated 52 cDNA clones of KRAB-ZFPs from U1 cell lines and screened them to identify which were capable of regulating HIV-1 gene expression. We identified 5 KRAB-ZFPs that suppressed ≥50\% of HIV-1 LTR. Of the 5 identified KRAB-ZFPs, the expression of ZNF10 significantly enhanced the transcriptional repression activity of the LTR compared with other ZNFs. In addition, the depletion of endogenous ZNF10 led to the activation of HIV-1 LTR. The repressor activity of ZNF10 was required for TRIM28, SETDB1 and HP1-gamma binding. These results indicate that ZNF10 could be involved in a potent intrinsic antiretroviral defense.",

author = "Hironori Nishitsuji and Leila Sawada and Ryuichi Sugiyama and Hiroshi Takaku",

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T1 - ZNF10 inhibits HIV-1 LTR activity through interaction with NF-κB and Sp1 binding motifs

AU - Nishitsuji, Hironori

AU - Sawada, Leila

AU - Sugiyama, Ryuichi

AU - Takaku, Hiroshi

PY - 2015/7/3

Y1 - 2015/7/3

N2 - Abstract Kruppel-associated box-containing zinc finger (KRAB-ZNF) genes constitute the single largest gene family of transcriptional repressors in the genomes of higher organisms. In this study, we isolated 52 cDNA clones of KRAB-ZFPs from U1 cell lines and screened them to identify which were capable of regulating HIV-1 gene expression. We identified 5 KRAB-ZFPs that suppressed ≥50% of HIV-1 LTR. Of the 5 identified KRAB-ZFPs, the expression of ZNF10 significantly enhanced the transcriptional repression activity of the LTR compared with other ZNFs. In addition, the depletion of endogenous ZNF10 led to the activation of HIV-1 LTR. The repressor activity of ZNF10 was required for TRIM28, SETDB1 and HP1-gamma binding. These results indicate that ZNF10 could be involved in a potent intrinsic antiretroviral defense.

AB - Abstract Kruppel-associated box-containing zinc finger (KRAB-ZNF) genes constitute the single largest gene family of transcriptional repressors in the genomes of higher organisms. In this study, we isolated 52 cDNA clones of KRAB-ZFPs from U1 cell lines and screened them to identify which were capable of regulating HIV-1 gene expression. We identified 5 KRAB-ZFPs that suppressed ≥50% of HIV-1 LTR. Of the 5 identified KRAB-ZFPs, the expression of ZNF10 significantly enhanced the transcriptional repression activity of the LTR compared with other ZNFs. In addition, the depletion of endogenous ZNF10 led to the activation of HIV-1 LTR. The repressor activity of ZNF10 was required for TRIM28, SETDB1 and HP1-gamma binding. These results indicate that ZNF10 could be involved in a potent intrinsic antiretroviral defense.

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ER -

ZNF10 inhibits HIV-1 LTR activity through interaction with NF-κB and Sp1 binding motifs

Abstract

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