ZNF265 - A novel spliceosomal protein able to induce alternative splicing

David J. Adams, Louise Van Der Weyden, Akira Maeda, Stefan Stamm, Brian J. Morris, John E.J. Rasko

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

The formation of the active spliceosome, its recruitment to active areas of transcription, and its role in pre-mRNA splicing depends on the association of a number of multifunctional serine/arginine-rich (SR) proteins. ZNF265 is an arginine/serine-rich (RS) domain containing zinc finger protein with conserved pre-mRNA splicing protein motifs. Here we show that ZNF265 immunoprecipitates from splicing extracts in association with mRNA, and that it is able to alter splicing patterns of Tra2-β1 transcripts in a dose-dependent manner in HEK 293 cells. Yeast two-hybrid analysis and immunoprecipitation indicated interaction of ZNF265 with the essential splicing factor proteins U1-70K and U2AF35. Confocal microscopy demonstrated colocalization of ZNF265 with the motor neuron gene product SMN, the snRNP protein U1-70K, the SR protein SC35, and with the transcriptosomal components p300 and YY1. Transfection of HT-1080 cells with ZNF265-EGFP fusion constructs showed that nuclear localization of ZNF265 required the RS domain. Alignment with other RS domain-containing proteins revealed a high degree of SR dipeptide conservation. These data show that ZNF265 functions as a novel component of the mRNA processing machinery.

Original languageEnglish
Pages (from-to)25-32
Number of pages8
JournalJournal of Cell Biology
Volume154
Issue number1
DOIs
Publication statusPublished - 09-07-2001

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Alternative Splicing
Serine
Arginine
RNA Precursors
Proteins
U1 Small Nuclear Ribonucleoproteins
Spliceosomes
P300 Event-Related Potentials
Messenger RNA
Amino Acid Motifs
Dipeptides
HEK293 Cells
Zinc Fingers
Motor Neurons
Immunoprecipitation
Confocal Microscopy
Transfection
Yeasts
Genes

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Adams, D. J., Van Der Weyden, L., Maeda, A., Stamm, S., Morris, B. J., & Rasko, J. E. J. (2001). ZNF265 - A novel spliceosomal protein able to induce alternative splicing. Journal of Cell Biology, 154(1), 25-32. https://doi.org/10.1083/jcb.200010059
Adams, David J. ; Van Der Weyden, Louise ; Maeda, Akira ; Stamm, Stefan ; Morris, Brian J. ; Rasko, John E.J. / ZNF265 - A novel spliceosomal protein able to induce alternative splicing. In: Journal of Cell Biology. 2001 ; Vol. 154, No. 1. pp. 25-32.
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Adams, DJ, Van Der Weyden, L, Maeda, A, Stamm, S, Morris, BJ & Rasko, JEJ 2001, 'ZNF265 - A novel spliceosomal protein able to induce alternative splicing', Journal of Cell Biology, vol. 154, no. 1, pp. 25-32. https://doi.org/10.1083/jcb.200010059

ZNF265 - A novel spliceosomal protein able to induce alternative splicing. / Adams, David J.; Van Der Weyden, Louise; Maeda, Akira; Stamm, Stefan; Morris, Brian J.; Rasko, John E.J.

In: Journal of Cell Biology, Vol. 154, No. 1, 09.07.2001, p. 25-32.

Research output: Contribution to journalArticle

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