β-globin transcripts carrying a single intron with three adjacent nucleotides of 5′ exon are efficiently spliced in vitro irrespective of intron position or surrounding exon sequences

Akila Mayeda, Yasumi Ohshima

研究成果: Article査読

11 被引用数 (Scopus)

抄録

To examine the role of exon sequences and intron position in the splicing of an mRNA precursor, we prepared series of sense or anti-sense transcripts of human β-globin cDNA in which a cassette containing the β-globin first intron was inserted into one of seven unusual positions. The intron cassette consisted of the intron alone (MI), the intron with three adjacent base pairs of the 5′ exon (MI), or the intron with both 5′ and 3′ exon sequences. All these transcripts were examined in an in vitro splicing system with a HeLa cell nuclear extract. The sense transcripts carrying MI cassette were spliced efficiently and independently of the intron position, except when the 3′ exon was too short. The anti-sense transcripts carrying MI cassette produced significantly less spliced products than did those of the sense transcripts. This was mostly because of the instability of the anti-sense transcripts, and the actual splicing efficiency was similar to that seen in the sense transcripts. Sense or anti-sense transcripts carrying MI cassette were spliced to various extents depending on the surrounding sequences. The results indicate that only three nucleotides of the 5′ exon are required as specific exon sequences in the splicing of an mRNA precursor carrying a single intron, and that the intron position does not significantly affect the splicing efficiency in vitro.

本文言語English
ページ(範囲)4671-4676
ページ数6
ジャーナルNucleic acids research
18
16
DOI
出版ステータスPublished - 25-08-1990
外部発表はい

All Science Journal Classification (ASJC) codes

  • 遺伝学

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