16-kilodalton rice protein is one of the major allergens in rice grain extract and responsible for cross-allergenicity between cereal grains in the poaceae family

Atsuo Urisu, Kazue Yamada, Susumu Masuda, Hidekatsu Komada, Eiko Wada, Yasuto Kondo, Famiya Horiba, Mitsutoshi Tsuruta, Takehiko Yasaki, Masanori Yamada, Shinpei Torii, Ryo Nakamura

研究成果: ジャーナルへの寄稿学術論文査読

87 被引用数 (Scopus)

抄録

Cross-allergenicity between five cereal grains including rice, wheat, corn, Japanese millet (Panicum crus-galli L. var. frumentaceum Trin.) and Italian millet (Setaria italica Beauv. var. germanica schrad.) was examined by radioallergosorbent test (RAST) and RAST inhibition assay. There were significant close correlations between every combinations of RAST values for the five cereal grain extracts. RAST inhibition assay of each extract against RAST discs coupled with other cereal grain extracts indicated marked cross-reactivity of IgE binding between these cereal grain extracts. Rice protein 16KD (RPI6KD) was shown to be one of major allergens in rice grain extracts by immunoblotting analysis, histamine release assay from human leukocytes and RAST inhibition. Next, the involvement of RP16KD in the cross-allergenicity between these cereals was investigated. RAST values for RP16KD significantly correlated with that for Italian millet as well as rice but not with those for corn and wheat. There was a trend of positive correlation between RAST values for RP16KD and Japanese millet. In the RAST inhibition assay using sera with positive RAST for these five cereal grain extracts and RP16KD, RP16KD inhibited IgE binding to these all cereal discs in a dose-dependent manner. Similarly, all of the five cereal grain extracts showed an effective decrease in IgE binding to the RPI6KD disc. These results indicated possible participation of IgE binding structure on RP16KD in cross-allergenicity between these cereal grain extracts in the Poaceae family.

本文言語英語
ページ(範囲)244-252
ページ数9
ジャーナルInternational Archives of Allergy and Immunology
96
3
DOI
出版ステータス出版済み - 1991

All Science Journal Classification (ASJC) codes

  • 免疫アレルギー学
  • 免疫学

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