16S ribosomal RNA methylation: Emerging resistance mechanism against aminoglycosides

Yohei Doi, Yoshichika Arakawa

研究成果: Review article

324 引用 (Scopus)

抄録

Methylation of 16S ribosomal RNA (rRNA) has recently emerged as a new mechanism of resistance against aminoglycosides among gram-negative pathogens belonging to the family Enterobacteriaceae and glucose-nonfermentative microbes, including Pseudomonas aeruginosa and Acinetobacter species. This event is mediated by a newly recognized group of 16S rRNA methylases, which share modest similarity to those produced by aminoglycoside-producing actinomycetes. Their presence confers a high level of resistance to all parenterally administered aminoglycosides that are currently in clinical use. The responsible genes are mostly located on transposons within transferable plasmids, which provides them with the potential to spread horizontally and may in part explain the already worldwide distribution of this novel resistance mechanism. Some of these organisms have been found to coproduce extended-spectrum β-lactamases or metallo-β-lactamases, contributing to their multidrug-resistant phenotypes. A 2-tiered approach, consisting of disk diffusion tests followed by confirmation with polymerase chain reaction, is recommended for detection of 16S rRNA methylase-mediated resistance.

元の言語English
ページ(範囲)88-94
ページ数7
ジャーナルClinical Infectious Diseases
45
発行部数1
DOI
出版物ステータスPublished - 01-07-2007

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Aminoglycosides
16S Ribosomal RNA
Methylation
Acinetobacter
Actinobacteria
Enterobacteriaceae
Pseudomonas aeruginosa
Plasmids
Phenotype
Glucose
Polymerase Chain Reaction
Genes
rRNA (adenosine-O-2'-)methyltransferase

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases

これを引用

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16S ribosomal RNA methylation : Emerging resistance mechanism against aminoglycosides. / Doi, Yohei; Arakawa, Yoshichika.

:: Clinical Infectious Diseases, 巻 45, 番号 1, 01.07.2007, p. 88-94.

研究成果: Review article

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