抄録
The adenomatous polyposis coli (APC) is a multifunctional protein as well as a tumor suppressor. To determine the functions of the C-terminal domain of APC, we explored APC 1638T/1638T (APC1638T) mice that express a truncated APC lacking the C-terminal domain. The APC1638T mice were tumor free and exhibited growth retardation. In the present study, we compared small intestinal crypt-villus cells homeostasis in APC +/+ (WT) mice and APC1638T mice. The body weight of APC1638T mice was significantly smaller than that of WT mice at all ages. The length of small intestine of APC1638T mice was significantly shorter than that of WT mice. The crypt-villus axis was significantly elongated, and the number of intestinal epithelial cells also increased in APC1638T mice compared with those in WT mice. However, the number of intestinal epithelial cells per 100 µm of villi was not different between WT and APC1638T mice. Migration and proliferation of intestinal epithelial cells in APC1638T mice were faster than that in WT mice. The population of Goblet cells, Paneth cells, and enteroendocrine cells was significantly altered in APC1638T mice. These results indicate that C-terminal domain of APC has a role in the regulation of intestinal epithelium homeostasis.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 94-102 |
| ページ数 | 9 |
| ジャーナル | Medical Molecular Morphology |
| 巻 | 50 |
| 発行部数 | 2 |
| DOI | |
| 出版物ステータス | Published - 01-06-2017 |
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All Science Journal Classification (ASJC) codes
- Pathology and Forensic Medicine
- Molecular Biology
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A disturbance of intestinal epithelial cell population and kinetics in APC1638T mice. / Wang, Tuya; Onouchi, Takanori; Yamada, Nami O.; Matsuda, Shuji; Senda, Takao.
：: Medical Molecular Morphology, 巻 50, 番号 2, 01.06.2017, p. 94-102.研究成果: Article
TY - JOUR
T1 - A disturbance of intestinal epithelial cell population and kinetics in APC1638T mice
AU - Wang, Tuya
AU - Onouchi, Takanori
AU - Yamada, Nami O.
AU - Matsuda, Shuji
AU - Senda, Takao
PY - 2017/6/1
Y1 - 2017/6/1
N2 - The adenomatous polyposis coli (APC) is a multifunctional protein as well as a tumor suppressor. To determine the functions of the C-terminal domain of APC, we explored APC 1638T/1638T (APC1638T) mice that express a truncated APC lacking the C-terminal domain. The APC1638T mice were tumor free and exhibited growth retardation. In the present study, we compared small intestinal crypt-villus cells homeostasis in APC +/+ (WT) mice and APC1638T mice. The body weight of APC1638T mice was significantly smaller than that of WT mice at all ages. The length of small intestine of APC1638T mice was significantly shorter than that of WT mice. The crypt-villus axis was significantly elongated, and the number of intestinal epithelial cells also increased in APC1638T mice compared with those in WT mice. However, the number of intestinal epithelial cells per 100 µm of villi was not different between WT and APC1638T mice. Migration and proliferation of intestinal epithelial cells in APC1638T mice were faster than that in WT mice. The population of Goblet cells, Paneth cells, and enteroendocrine cells was significantly altered in APC1638T mice. These results indicate that C-terminal domain of APC has a role in the regulation of intestinal epithelium homeostasis.
AB - The adenomatous polyposis coli (APC) is a multifunctional protein as well as a tumor suppressor. To determine the functions of the C-terminal domain of APC, we explored APC 1638T/1638T (APC1638T) mice that express a truncated APC lacking the C-terminal domain. The APC1638T mice were tumor free and exhibited growth retardation. In the present study, we compared small intestinal crypt-villus cells homeostasis in APC +/+ (WT) mice and APC1638T mice. The body weight of APC1638T mice was significantly smaller than that of WT mice at all ages. The length of small intestine of APC1638T mice was significantly shorter than that of WT mice. The crypt-villus axis was significantly elongated, and the number of intestinal epithelial cells also increased in APC1638T mice compared with those in WT mice. However, the number of intestinal epithelial cells per 100 µm of villi was not different between WT and APC1638T mice. Migration and proliferation of intestinal epithelial cells in APC1638T mice were faster than that in WT mice. The population of Goblet cells, Paneth cells, and enteroendocrine cells was significantly altered in APC1638T mice. These results indicate that C-terminal domain of APC has a role in the regulation of intestinal epithelium homeostasis.
UR - http://www.scopus.com/inward/record.url?scp=85008689595&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85008689595&partnerID=8YFLogxK
U2 - 10.1007/s00795-016-0152-5
DO - 10.1007/s00795-016-0152-5
M3 - Article
C2 - 28070680
AN - SCOPUS:85008689595
VL - 50
SP - 94
EP - 102
JO - Medical Electron Microscopy
JF - Medical Electron Microscopy
SN - 0918-4287
IS - 2
ER -