A herpesvirus specific motif of Epstein-Barr virus DNA polymerase is required for the efficient lytic genome synthesis

Yohei Narita, Atsuko Sugimoto, Daisuke Kawashima, Takahiro Watanabe, Teru Kanda, Hiroshi Kimura, Tatsuya Tsurumi, Takayuki Murata

研究成果: Article

4 引用 (Scopus)

抄録

Epstein-Barr virus (EBV) is associated with several malignancies, including Burkitt lymphoma and nasopharyngeal carcinoma. To overcome such disorders, understanding the molecular mechanisms of the EBV replication is important. The EBV DNA polymerase (Pol) is one of the essential factors for viral lytic DNA replication. Although it is well known that its C-terminal half, possessing DNA polymerase and 3′-5′ exonuclease activity, is highly conserved among Family B Pols, the NH 2 -terminal half has yet to be characterized in detail. In this study, we show that a stretch of hydrophobic amino acids within the pre-NH 2 -terminal domain of EBV Pol plays important role. In addition, we could identify the most essential residue for replication in the motif. These findings will shed light on molecular mechanisms of viral DNA synthesis and will help to develop new herpesviruses treatments.

元の言語English
記事番号11767
ジャーナルScientific reports
5
DOI
出版物ステータスPublished - 30-06-2015

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Herpesviridae
Human Herpesvirus 4
Viral DNA
Genome
Exonucleases
Burkitt Lymphoma
DNA-Directed DNA Polymerase
Virus Replication
DNA Replication
Amino Acids
Epstein-barr virus BALF5 protein
Neoplasms

All Science Journal Classification (ASJC) codes

  • General

これを引用

Narita, Yohei ; Sugimoto, Atsuko ; Kawashima, Daisuke ; Watanabe, Takahiro ; Kanda, Teru ; Kimura, Hiroshi ; Tsurumi, Tatsuya ; Murata, Takayuki. / A herpesvirus specific motif of Epstein-Barr virus DNA polymerase is required for the efficient lytic genome synthesis. :: Scientific reports. 2015 ; 巻 5.
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abstract = "Epstein-Barr virus (EBV) is associated with several malignancies, including Burkitt lymphoma and nasopharyngeal carcinoma. To overcome such disorders, understanding the molecular mechanisms of the EBV replication is important. The EBV DNA polymerase (Pol) is one of the essential factors for viral lytic DNA replication. Although it is well known that its C-terminal half, possessing DNA polymerase and 3′-5′ exonuclease activity, is highly conserved among Family B Pols, the NH 2 -terminal half has yet to be characterized in detail. In this study, we show that a stretch of hydrophobic amino acids within the pre-NH 2 -terminal domain of EBV Pol plays important role. In addition, we could identify the most essential residue for replication in the motif. These findings will shed light on molecular mechanisms of viral DNA synthesis and will help to develop new herpesviruses treatments.",
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A herpesvirus specific motif of Epstein-Barr virus DNA polymerase is required for the efficient lytic genome synthesis. / Narita, Yohei; Sugimoto, Atsuko; Kawashima, Daisuke; Watanabe, Takahiro; Kanda, Teru; Kimura, Hiroshi; Tsurumi, Tatsuya; Murata, Takayuki.

:: Scientific reports, 巻 5, 11767, 30.06.2015.

研究成果: Article

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AU - Narita, Yohei

AU - Sugimoto, Atsuko

AU - Kawashima, Daisuke

AU - Watanabe, Takahiro

AU - Kanda, Teru

AU - Kimura, Hiroshi

AU - Tsurumi, Tatsuya

AU - Murata, Takayuki

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AB - Epstein-Barr virus (EBV) is associated with several malignancies, including Burkitt lymphoma and nasopharyngeal carcinoma. To overcome such disorders, understanding the molecular mechanisms of the EBV replication is important. The EBV DNA polymerase (Pol) is one of the essential factors for viral lytic DNA replication. Although it is well known that its C-terminal half, possessing DNA polymerase and 3′-5′ exonuclease activity, is highly conserved among Family B Pols, the NH 2 -terminal half has yet to be characterized in detail. In this study, we show that a stretch of hydrophobic amino acids within the pre-NH 2 -terminal domain of EBV Pol plays important role. In addition, we could identify the most essential residue for replication in the motif. These findings will shed light on molecular mechanisms of viral DNA synthesis and will help to develop new herpesviruses treatments.

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