A liquid‐phase binding analysis for L‐selectin: A strong dependency on highly clustered sulfate groups

Tomoaki YOSHIDA, Christopher FENNIE, Laurence A. LASKY, Yuan C. LEE

研究成果: Article査読

20 被引用数 (Scopus)

抄録

L‐selectin, together with E‐ and P‐selectins, forms a newer group of cell adhesion molecules which are believed to interact with carbohydrate ligands [Lasky, L. A., Singer, M. S., Yednoch, T. A., Dowbenko, D., Fennie, C., Rodriguez, H., Nguyen, T., Stachel, S. & Rosen, S. D. (1989) Cell 56, 1045–1055]. Using radiolabeled fucoidan as a reference ligand, we have developed a new liquid‐phase microcentrifugation assay where fine differences in binding affinity can be compared accurately. We found that glucan sulfates strongly inhibited the binding of fucoidan by murine L‐selectin‐IgG chimera. The efficacy of inhibition is extremely dependent on the size (up to 12 kDa) and the sulfate density (up to two sulfate groups/glucose molecule) of the glucan sulfates. The nature of the inter‐glucose linkages is also important. These data suggest that the binding by L‐selectin prefers certain clustering and proper spatial arrangement of the anionic groups.

本文言語English
ページ(範囲)703-709
ページ数7
ジャーナルEuropean Journal of Biochemistry
222
2
DOI
出版ステータスPublished - 06-1994
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学

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