A monoclonal antibody (8H3) that binds to rat T lineage cells and augments in vitro proliferative responses

Yoshihiro Torimoto, Miyuki Kinebuchi, Akihiro Matsuura, Kokichi Kikuchi, Toshimitsu Uede

研究成果: Article

10 引用 (Scopus)

抄録

A murine monoclonal antibody, designated 8113, recognizes a cell surface antigen expressed exclusively on rat T lineage cells. 8113 antibody immunoprecipitated 180-, 120-, and 90-kD components from rat thymorytes as well as splenic T cells under nonreducing conditions. 8113 antibody specifically inhibited the binding of thymocytes to fibronectin. Furthermore, binding of rat thymocytes to immobilized synthetic peptide GlyArg-Gly-Asp-Ser-Pro-Cys-BSA was inhibited by 8113 antibody as was GlyArg-Gly-Asp-Ser-Pro-Cys, but not by Gly-ArgAla-Asp-Ser-Pro-Lys or Gly-Arg-Gly-Glu-Ser-Pro. Crosslinking of SH3 antigen on double-negative thymocytes and adult thymocytes, as well as splenic T lymphocytes by 8113 antibody and F(ab')2 fragments of goat antibodies to mouse immunoglobulin, led to an increase in the concentration ofcytoplasmic free Ca2+ due to the release of Ca2+ from intracellular stores as well as the influx of Ca2+ from extracellular sources. Expression of interleukin 2 receptor and subsequently cell proliferation was observed upon incubation of thymocytes and splenic T cells with 8113 antibody. Furthermore, 8113 antibody induced the proliferation of double-negative thymocytes. These data collectively indicate that a cell surface antigen, 8113, is involved in not only cell adhesion but also involved in the expression of immature as well as mature thymocytes.

元の言語English
ページ(範囲)1315-1323
ページ数9
ジャーナルJournal of Experimental Medicine
172
発行部数5
DOI
出版物ステータスPublished - 01-11-1990

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Thymocytes
Monoclonal Antibodies
Antibodies
Surface Antigens
glycyl-arginyl-glycyl-glutamyl-seryl-proline
T-Lymphocytes
Immunoglobulin Fragments
Interleukin-2 Receptors
In Vitro Techniques
Fibronectins
Goats
Cell Adhesion
Immunoglobulins
Cell Proliferation
Antigens
Peptides

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

これを引用

Torimoto, Yoshihiro ; Kinebuchi, Miyuki ; Matsuura, Akihiro ; Kikuchi, Kokichi ; Uede, Toshimitsu. / A monoclonal antibody (8H3) that binds to rat T lineage cells and augments in vitro proliferative responses. :: Journal of Experimental Medicine. 1990 ; 巻 172, 番号 5. pp. 1315-1323.
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abstract = "A murine monoclonal antibody, designated 8113, recognizes a cell surface antigen expressed exclusively on rat T lineage cells. 8113 antibody immunoprecipitated 180-, 120-, and 90-kD components from rat thymorytes as well as splenic T cells under nonreducing conditions. 8113 antibody specifically inhibited the binding of thymocytes to fibronectin. Furthermore, binding of rat thymocytes to immobilized synthetic peptide GlyArg-Gly-Asp-Ser-Pro-Cys-BSA was inhibited by 8113 antibody as was GlyArg-Gly-Asp-Ser-Pro-Cys, but not by Gly-ArgAla-Asp-Ser-Pro-Lys or Gly-Arg-Gly-Glu-Ser-Pro. Crosslinking of SH3 antigen on double-negative thymocytes and adult thymocytes, as well as splenic T lymphocytes by 8113 antibody and F(ab')2 fragments of goat antibodies to mouse immunoglobulin, led to an increase in the concentration ofcytoplasmic free Ca2+ due to the release of Ca2+ from intracellular stores as well as the influx of Ca2+ from extracellular sources. Expression of interleukin 2 receptor and subsequently cell proliferation was observed upon incubation of thymocytes and splenic T cells with 8113 antibody. Furthermore, 8113 antibody induced the proliferation of double-negative thymocytes. These data collectively indicate that a cell surface antigen, 8113, is involved in not only cell adhesion but also involved in the expression of immature as well as mature thymocytes.",
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A monoclonal antibody (8H3) that binds to rat T lineage cells and augments in vitro proliferative responses. / Torimoto, Yoshihiro; Kinebuchi, Miyuki; Matsuura, Akihiro; Kikuchi, Kokichi; Uede, Toshimitsu.

:: Journal of Experimental Medicine, 巻 172, 番号 5, 01.11.1990, p. 1315-1323.

研究成果: Article

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T1 - A monoclonal antibody (8H3) that binds to rat T lineage cells and augments in vitro proliferative responses

AU - Torimoto, Yoshihiro

AU - Kinebuchi, Miyuki

AU - Matsuura, Akihiro

AU - Kikuchi, Kokichi

AU - Uede, Toshimitsu

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