TY - JOUR
T1 - A morphological study of adipose-derived stem cell sheets created with temperature-responsive culture dishes using scanning electron microscopy
AU - Taki, Yasuhiko
AU - Fuku, Atsushi
AU - Nakamura, Yuka
AU - Koya, Terutsugu
AU - Kitajima, Hironori
AU - Tanida, Ikuhiro
AU - Takaki, Takashi
AU - Nozaki, Kaori
AU - Sunami, Hiroshi
AU - Hirata, Hiroaki
AU - Tachi, Yoshiyuki
AU - Shimasaki, Takeo
AU - Masauji, Togen
AU - Yamamoto, Naoki
AU - Ishigaki, Yasuhito
AU - Shimodaira, Shigetaka
AU - Shimizu, Yusuke
AU - Ichiseki, Toru
AU - Kaneuji, Ayumi
AU - Osawa, Satoshi
AU - Kawahara, Norio
N1 - Publisher Copyright:
© 2022, The Author(s) under exclusive licence to The Japanese Society for Clinical Molecular Morphology.
PY - 2022/9
Y1 - 2022/9
N2 - Adipose-derived stem cell (ADSC) sheets have potential to be effective in various therapies. In this study, we first demonstrated that a cell sheet composed of human ADSCs could be created using a new temperature-responsive culture dish from the DIC Corporation. The dish can cause detachment of adherent cells due to temperature changes, but a few morphological analyses have evaluated the presence or absence of damage on the detached surface of cell sheet. To characterize our ADSC sheet, we tried to observe the surface of ADSC sheets with scanning electron microscope (SEM) using the ionic liquid, which enables the rapid preparation of samples. No damage was found on the surface of the ADSC sheets on the side that had been in contact with the surface of the culture dishes. In addition, when the transcriptomes of the harvested cell sheets were compared with those of monolayer cultures, no up-regulation of cell death related genes were detected. These results propose that the detachment from temperature-responsive culture dish causes no serious damage on the prepared ADSC sheet. It is also suggested that the SEM with ionic liquids is a useful and rapid method for the analysis of ADSC sheets for therapy.
AB - Adipose-derived stem cell (ADSC) sheets have potential to be effective in various therapies. In this study, we first demonstrated that a cell sheet composed of human ADSCs could be created using a new temperature-responsive culture dish from the DIC Corporation. The dish can cause detachment of adherent cells due to temperature changes, but a few morphological analyses have evaluated the presence or absence of damage on the detached surface of cell sheet. To characterize our ADSC sheet, we tried to observe the surface of ADSC sheets with scanning electron microscope (SEM) using the ionic liquid, which enables the rapid preparation of samples. No damage was found on the surface of the ADSC sheets on the side that had been in contact with the surface of the culture dishes. In addition, when the transcriptomes of the harvested cell sheets were compared with those of monolayer cultures, no up-regulation of cell death related genes were detected. These results propose that the detachment from temperature-responsive culture dish causes no serious damage on the prepared ADSC sheet. It is also suggested that the SEM with ionic liquids is a useful and rapid method for the analysis of ADSC sheets for therapy.
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U2 - 10.1007/s00795-022-00319-8
DO - 10.1007/s00795-022-00319-8
M3 - Article
C2 - 35449367
AN - SCOPUS:85128525632
SN - 1860-1480
VL - 55
SP - 187
EP - 198
JO - Medical Molecular Morphology
JF - Medical Molecular Morphology
IS - 3
ER -