TY - JOUR
T1 - A murine Thy-1.2 reporter vector containing a SV40 origin for rapid cloning and analysis of eukaryotic promoters
AU - Kadokawa, Yuzo
AU - Kusakabe, Takahiro
AU - Kamachi, Yusuke
AU - Isobe, Ken ichi
AU - Kondoh, Hisato
AU - Ohyama, Takashi
PY - 1995/2/14
Y1 - 1995/2/14
N2 - A new vector, pATO, was constructed for rapid cloning and analysis of eukaryotic promoters. When a recombinant pATO, carrying a promoter sequence in its multiple cloning site, was introduced into COS cells, Thy-1.2 protein was produced on the cell surface, and was easily identified by an fluorescein-conjugated anti-Thy-1.2 antibody. The intensity of the fluorescence reflected the strength of the inserted promoter. Since pATO could replicate efficiently in COS cells, the recombinant plasmids recovered from a single COS cell were sufficient to transform Escherichia coli cells. This plasmid is applicable for the rapid and labor saving cloning of promoter elements.
AB - A new vector, pATO, was constructed for rapid cloning and analysis of eukaryotic promoters. When a recombinant pATO, carrying a promoter sequence in its multiple cloning site, was introduced into COS cells, Thy-1.2 protein was produced on the cell surface, and was easily identified by an fluorescein-conjugated anti-Thy-1.2 antibody. The intensity of the fluorescence reflected the strength of the inserted promoter. Since pATO could replicate efficiently in COS cells, the recombinant plasmids recovered from a single COS cell were sufficient to transform Escherichia coli cells. This plasmid is applicable for the rapid and labor saving cloning of promoter elements.
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U2 - 10.1016/0378-1119(94)00803-Z
DO - 10.1016/0378-1119(94)00803-Z
M3 - Article
C2 - 7875602
AN - SCOPUS:0028861649
SN - 0378-1119
VL - 153
SP - 277
EP - 278
JO - Gene
JF - Gene
IS - 2
ER -