TY - JOUR
T1 - A needle-type micro-sampling device for collecting nanoliter sap sample from plants
AU - Gao, Panpan
AU - Kasama, Toshihiro
AU - Godonoga, Maia
AU - Ogawa, Atsushi
AU - Sone, Chiharu
AU - Komine, Masashi
AU - Endo, Yoshishige
AU - Koide, Tetsushi
AU - Miyake, Ryo
N1 - Publisher Copyright:
© 2021, Springer-Verlag GmbH Germany, part of Springer Nature.
PY - 2021/5
Y1 - 2021/5
N2 - In plant research, measuring the physiological parameters of plants is vital for understanding the behavior and response of plants to changes in the external environment. Plant sap analysis provides an approach for elucidating the physiological condition of plants. However, to facilitate accurate sap analysis, a sampling device capable of collecting sap samples from plants is required. In this paper, a minimally invasive, needle-type micro-sampling device capable of collecting nanoliter (~ 91 nL) quantities of sap from plants is described. The developed micro-sampling system showed great reproducibility (3%) in experiments designed to assess sampling performance. As a proof of concept, sap samples were collected continuously from target plants with the micro-sampling system, and the dynamic changes in potassium ions, plant hormones and sugar levels inside plants were analyzed. The results demonstrated the feasibility of the micro-sampling device and its potential for developing a measurement system for plant research in the future.
AB - In plant research, measuring the physiological parameters of plants is vital for understanding the behavior and response of plants to changes in the external environment. Plant sap analysis provides an approach for elucidating the physiological condition of plants. However, to facilitate accurate sap analysis, a sampling device capable of collecting sap samples from plants is required. In this paper, a minimally invasive, needle-type micro-sampling device capable of collecting nanoliter (~ 91 nL) quantities of sap from plants is described. The developed micro-sampling system showed great reproducibility (3%) in experiments designed to assess sampling performance. As a proof of concept, sap samples were collected continuously from target plants with the micro-sampling system, and the dynamic changes in potassium ions, plant hormones and sugar levels inside plants were analyzed. The results demonstrated the feasibility of the micro-sampling device and its potential for developing a measurement system for plant research in the future.
KW - Dynamic measurements
KW - Micro-needle device
KW - Minimum invasive collection
KW - Onsite sampling device
KW - Quantitative sample manipulation
KW - Sample injector
UR - https://www.scopus.com/pages/publications/85102587392
UR - https://www.scopus.com/pages/publications/85102587392#tab=citedBy
U2 - 10.1007/s00216-021-03246-2
DO - 10.1007/s00216-021-03246-2
M3 - Article
C2 - 33733702
AN - SCOPUS:85102587392
SN - 1618-2642
VL - 413
SP - 3081
EP - 3091
JO - Analytical and Bioanalytical Chemistry
JF - Analytical and Bioanalytical Chemistry
IS - 11
ER -