A new gerbil model of hindbrain ischemia by extracranial occlusion of the bilateral vertebral arteries

Ryuji Hata, Masayasu Matsumoto, Kazuo Kitagawa, Tomohiro Matsuyama, Toshiho Ohtsuki, Masafumi Tagaya, Nobuo Handa, Michio Niinobe, Katsuhiko Mikoshiba, Tsunehiko Nishimura, Takehiko Yanagihara, Takenobu Kamada

研究成果: Article査読

8 被引用数 (Scopus)

抄録

A new gerbil model of hindbrain ischemia was induced by extracranial occlusion of the bilateral vertebral arteries just before their entry into the transverse foramen of the cervical vertebra. Carbon black studies, performed at 5 min after occlusion, revealed that the pons-medulla oblongata, and the cerebellum were quite ischemic in all animals. Cardiovascular changes in mean arterial blood pressure (MABP) and heart rate were recorded until 30 min after occlusion, and revealed that the typical cerebral ischemic response (i.e., abrupt increase in MABP, bradycardia, and apnea) was elicited in all animals (n = 10). Thirty minutes after occlusion, animals (n = 4) were decapitated and immersion-fixed. Brain sections were stained with hematoxylin-eosin (HE) and also immunostained for microtubule-associated protein 2 in order to evaluate ischemic neuronal damage from 30 min of ischemia. By HE staining, ischemic lesions were detected bilaterally in the oculomotor, the trigeminal motor, the lateral vestibular, and the cerebellar interpositus nucleus. In addition, immunostaining revealed ischemic lesions in several other hindbrain areas. In conclusion, we could successfully establish a new gerbil model of hindbrain ischemia. Carbon black perfusion and hemodynamic studies revealed that severe and reproducible hindbrain ischemia was produced. By histopathological examination, we could also clearly demonstrate symmetrical ischemic lesions in several hindbrain areas.

本文言語English
ページ(範囲)79-89
ページ数11
ジャーナルJournal of the Neurological Sciences
121
1
DOI
出版ステータスPublished - 01-1994
外部発表はい

All Science Journal Classification (ASJC) codes

  • Neurology
  • Clinical Neurology

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