A new isoform of human myosin phosphatase targeting/regulatory subunit (MYPT2): cDNA cloning, tissue expression, and chromosomal mapping

Masaki Fujioka, Nobuaki Takahashi, Hideharu Odai, Shigemasa Araki, Kazuhito Ichikawa, Jianhua Feng, Mamoo Nakamura, Kozo Kaibuchi, David J. Hartshorne, Takeshi Nakano, Masaaki Ito

研究成果: ジャーナルへの寄稿学術論文査読

71 被引用数 (Scopus)

抄録

Myosin phosphatase target subunit 1 (MYPT1), a subunit of myosin phosphatase, plays a pivotal role in the regulation of myosin phosphatase activity. Here we have cloned a novel isoform of MYPT1, termed MYPT2, from a human brain cDNA library screened with a cDNA fragment of rat MYPT1. Overlapping clones indicated an open reading frame of 3763 nucleotides and a predicted polypeptide of mass 110,398. Ankyrin repeats and leucine zipper motifs were identified for the sequences 57-316 and 956-982, respectively. Overall, the deduced amino acid sequence of MYPT2 was 61% identical to MYPT1. MYPT2 gene is transcribed abundantly in heart and skeletal muscle, while Western blots using an antibody specific for MYPT2 showed exclusive expression of MYPT2 in heart and brain. A recombinant of the N-terminal two- thirds of MYPT2 bound to the catalytic subunit of type 1 phosphatase (δ isoform) and increased activity toward phosphorylated myosin light chain. In situ hybridization localized the human MYPT2 gene on chromosome 1q32.1, compared to the chromosomal location 12q15-q21.2 for MYPT1. It is suggested that the products of the two gene families of myosin phosphatase target subunit may be localized differently among various tissues.

本文言語英語
ページ(範囲)59-68
ページ数10
ジャーナルGenomics
49
1
DOI
出版ステータス出版済み - 01-04-1998
外部発表はい

All Science Journal Classification (ASJC) codes

  • 遺伝学

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