TY - JOUR
T1 - A new retrovirus produced by tissue culture cell line from mammary tumor of a house musk shrew, Suncus murinus
AU - Tsutsui, Yoshihiro
AU - Nagayoshi, Shoichi
AU - Saga, Shinsuke
AU - Takahashi, Masahide
AU - Aoyama, Atsuko
AU - Malavasi, Jollyanna Yamashiro
AU - Hayakawa, Seijun
AU - Yokoi, Toyoharu
AU - Oda, Sen Ichi
AU - Hoshino, Munemitsu
N1 - Funding Information:
We thank Professor K. Kondo for his constant advice and encouragement.W e also thank Dr. K. Ono, Dr. S. Yoshida, and Dr. Y. Nagai for their criticism during the course of this research. Our thanks are also due to Mr. J. Aoki, Miss M. Ito, Mr. K. Imaizumi, and Ms. F. Iwasa for their technical help, and to Mr. Smith for his help in preparing the manuscript. This research was supported in part by grants-in-aid for cancer research to M. Hoshino (57010040,5 9010043,5 7010089)a nd to S. Saga (59010031a),n d a grant-in-aid for scientific research to M. Hoshino (537008)f rom the Ministry of Education, Science and Culture of Japan.
PY - 1985/7/15
Y1 - 1985/7/15
N2 - A new type of retrovirus (Sm-MTV) released by cultured cells of a spontaneous mammary tumor from a house musk shrew, Suncus murinus, is described. The Sm-MTV is distinct morphologically from type C particles. In spite of certain morphological similarities to type B and type D retroviruses, the Sm-MTV is readily distinguishable. The extracellular virions had a spikeless envelope containing a centrally located nucleoid with a small electron-dense core surrounded by an inner membrane. The budding particles contained a doughnut-shaped nucleoid. Intracytoplasmic type A particles similar in profile to those associated with mouse mammary tumor cells were also found, and tended to form a small cluster of several particles in the cytoplasm. The virus banded at 1.169 g/cm3 in isopycnic centrifugation and possessed constitutive Mg2+-dependent reverse transcriptase. The viral RNA had a molecular size ranging from 50 to 70 S in its native form and 30 to 40 S in its denatured form by a glycerol gradient ultracentrifugation. Major viral polypeptides were 72K, 69K, 47K, 44K/43K, 27K, 20.5K, and 15K.
AB - A new type of retrovirus (Sm-MTV) released by cultured cells of a spontaneous mammary tumor from a house musk shrew, Suncus murinus, is described. The Sm-MTV is distinct morphologically from type C particles. In spite of certain morphological similarities to type B and type D retroviruses, the Sm-MTV is readily distinguishable. The extracellular virions had a spikeless envelope containing a centrally located nucleoid with a small electron-dense core surrounded by an inner membrane. The budding particles contained a doughnut-shaped nucleoid. Intracytoplasmic type A particles similar in profile to those associated with mouse mammary tumor cells were also found, and tended to form a small cluster of several particles in the cytoplasm. The virus banded at 1.169 g/cm3 in isopycnic centrifugation and possessed constitutive Mg2+-dependent reverse transcriptase. The viral RNA had a molecular size ranging from 50 to 70 S in its native form and 30 to 40 S in its denatured form by a glycerol gradient ultracentrifugation. Major viral polypeptides were 72K, 69K, 47K, 44K/43K, 27K, 20.5K, and 15K.
UR - https://www.scopus.com/pages/publications/0021931654
UR - https://www.scopus.com/pages/publications/0021931654#tab=citedBy
U2 - 10.1016/0042-6822(85)90324-1
DO - 10.1016/0042-6822(85)90324-1
M3 - Article
C2 - 4060590
AN - SCOPUS:0021931654
SN - 0042-6822
VL - 144
SP - 273
EP - 278
JO - Virology
JF - Virology
IS - 1
ER -