A new TEM-derived extended-spectrum β-lactamase (TEM-91) with an R164C substitution at the ω-loop confers ceftazidime resistance

Hiroshi Kurokawa; Naohiro Shibata; Yohei Doi; Keigo Shibayama; Kazunari Kamachi; Tetsuya Yagi; Yoshichika Arakawa

doi:10.1128/AAC.47.9.2981-2983.2003

A new TEM-derived extended-spectrum β-lactamase (TEM-91) with an R164C substitution at the ω-loop confers ceftazidime resistance

Hiroshi Kurokawa
, Naohiro Shibata
, Yohei Doi
, Keigo Shibayama
, Kazunari Kamachi
, Tetsuya Yagi
, Yoshichika Arakawa

研究成果: ジャーナルへの寄稿 › 学術論文 › 査読

12 被引用数 (Scopus)

抄録

A new plasmid-mediated TEM-derived extended-spectrum β-lactamase, TEM-91, was identified in a ceftazidime-resistant (MIC, >128 μg per ml) Escherichia coli strain isolated in 1996 in Japan. TEM-91 has three amino acid substitutions, R164C, M184T, and E240K, compared with TEM-1 penicillinase. The isoelectric point (pI), K_m, and k_cat of TEM-91 for ceftazidime were 5.7, 179 μM, and 29.0 s^-1, respectively. The K_i of clavulanic acid for ceftazidime hydrolysis was 30.3 nM.

本文言語	英語
ページ（範囲）	2981-2983
ページ数	3
ジャーナル	Antimicrobial agents and chemotherapy
巻	47
号	9
DOI	https://doi.org/10.1128/AAC.47.9.2981-2983.2003
出版ステータス	出版済み - 01-09-2003
外部発表	はい

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All Science Journal Classification (ASJC) codes

薬理学
薬理学（医学）
感染症

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引用スタイル

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abstract = "A new plasmid-mediated TEM-derived extended-spectrum β-lactamase, TEM-91, was identified in a ceftazidime-resistant (MIC, >128 μg per ml) Escherichia coli strain isolated in 1996 in Japan. TEM-91 has three amino acid substitutions, R164C, M184T, and E240K, compared with TEM-1 penicillinase. The isoelectric point (pI), Km, and kcat of TEM-91 for ceftazidime were 5.7, 179 μM, and 29.0 s-1, respectively. The Ki of clavulanic acid for ceftazidime hydrolysis was 30.3 nM.",

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AU - Doi, Yohei

AU - Shibayama, Keigo

AU - Kamachi, Kazunari

AU - Yagi, Tetsuya

AU - Arakawa, Yoshichika

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