A novel apoptosis-inducing protein from Helicobacter pylori

Keigo Shibayama, Kazunari Kamachi, Noriyo Nagata, Tetsuya Yagi, Toshi Nada, Yohei Doi, Naohiro Shibata, Keiko Yokoyama, Kunikazu Yamane, Haru Kato, Yoshitsugu Iinuma, Yoshichika Arakawa

研究成果: ジャーナルへの寄稿学術論文査読

22 被引用数 (Scopus)


Helicobacter pylori infection induces apoptosis in gastric epithelial cells. Here, we report a novel apoptosis-inducing protein that functions as a leading factor in H. pylori-mediated apoptosis induction. We purified the protein from H. pylori by separating fractions that showed apoptosis-inducing activity. This protein induced apoptosis of AGS cells in a dose-dependent manner. The purified protein consisted of two protein fragments with molecular masses of about 40 and 22 kDa, which combined to constitute a single complex in their natural form. N-terminal sequencing indicated that both these protein fragments were encoded by the HP1118 gene. The purified protein exhibited γ-glutamyl transpeptidase activity, the inhibition of which by 6-diazo-5-oxo-L-norleucine resulted in a complete loss of apoptosis-inducing activity. To the best of our knowledge, the apoptosis-inducing function is a newly identified physiological role for bacterial γ-glutamyl transpeptidase. The apoptosis-inducing activity of the isogenic mutant γ-glutamyl transpeptidase-deficient strain was significantly lower compared with that of the parent strain, demonstrating that γ-glutamyl transpeptidase plays a significant role in H. pylori-mediated apoptosis. Our findings provide new insights into H. pylori pathogenicity and reveal a novel aspect of the bacterial γ-glutamyl transpeptidase function.

ジャーナルMolecular Microbiology
出版ステータス出版済み - 2003

All Science Journal Classification (ASJC) codes

  • 微生物学
  • 分子生物学


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