A Novel Glial Growth Inhibitory Factor, Gliostatin, Derived from Neurofibroma

Kiyofumi Asai, Takayoshi Hirano, Shuji Kaneko, Akihiko Moriyama, Keiko Nakanishi, Ichiro Isobe, Yaman Z. Eksioglu, Taiji Kato

研究成果: Article査読

49 被引用数 (Scopus)

抄録

Neurofibroma tissue was investigated for the presence of glial growth modulators that would suppress the proliferation of glial cells. A novel endogenous polypeptide inhibitor of proliferation and DNA synthesis in glial cells, gliostatin, was purified from the extracts of neurofibroma by a procedure comprising dye and anion‐exchange column chromatography, and HPLC. A monoclonal antibody raised against partially purified gliostatin showed no cross‐reactivity with known cytokines, but adsorbed the growth inhibitory activity of gliostatin and immunochemi‐cally visualized the putative gliostatin bands on western blot analyses. Although the product showed an apparent Mr of 100,000 accompanied by an inhibitory activity on gel filtration column chromatography, it migrated at a lower apparent Mr of 50,000 under the reducing conditions on western blotting, indicating that a homodimeric structure of native gliostatin consisted of 50‐kDa subcomponents. Gliostatin was a potent growth inhibitor acting at nanomolar concentrations against all glial tumor cells and glia maturation factor‐stimulated astroblasts, but not neuronal cells.

本文言語English
ページ(範囲)307-317
ページ数11
ジャーナルJournal of neurochemistry
59
1
DOI
出版ステータスPublished - 07-1992
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学
  • 細胞および分子神経科学

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