A novel heterotetrameric structure of the crenarchaeal PCNA2-PCNA3 complex

Akito Kawai, Hiroshi Hashimoto, Shigesada Higuchi, Masaru Tsunoda, Mamoru Sato, Kazuo T. Nakamura, Shuichi Miyamoto

研究成果: Article査読

10 被引用数 (Scopus)


Proliferating cell nuclear antigen (PCNA) is a key protein that orchestrates the arrangement of DNA-processing proteins on DNA during DNA metabolism. In crenarchaea, PCNA forms a heterotrimer (PCNA123) consisting of PCNA1, PCNA2, and PCNA3, while in most eukaryotes and many archaea PCNAs form a homotrimer. Interestingly, unique oligomeric PCNAs from Sulfolobus tokodaii were reported in which PCNA2 and PCNA3 form a heterotrimer without PCNA1. In this paper, we describe the crystal structure of the stoPCNA2- stoPCNA3 complex. While most DNA sliding clamps form ring-shaped structures, our crystal structure showed an elliptic ring-like heterotetrameric complex, differing from a previous reports. Furthermore, we investigated the composition and the dimension of the stoPCNA2- stoPCNA3 complex in the solution using gel-filtration column chromatography and small-angle X-ray scattering analyses, respectively. These results indicate that stoPCNA2 and stoPCNA3 form the heterotetramer in solution. Based on our heterotetrameric structure, we propose a possible biological role for the heterotetrameric complex as a Holliday junction clamp.

ジャーナルJournal of Structural Biology
出版ステータスPublished - 06-2011

All Science Journal Classification (ASJC) codes

  • 構造生物学


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