A novel minor histocompatibility antigen recognized by HLA-A31 restricted cytotoxic T lymphocytes generated from HLA-identical bone marrow donor lymphocytes

M. Yazaki, T. Takahashi, M. Andho, Yoshiki Akatsuka, Tetsuya Ito, Y. Miyake, Y. Ito, S. Nakamura, Y. Wada

研究成果: Article

12 引用 (Scopus)

抄録

Bulk cytotoxic T lymphocytes (CTL) were generated by in vitro stimulation of BMT donor lymphocytes with Philadelphia chromosome (Ph)-positive leukemic cells from an HLA-identical sibling patient. CTL were cytotoxic against the patient's leukemic cells as well as the EBV-lymphoblastoid cell line (EBV-LCL) generated from the patient's cells, suggesting that they recognize a minor histocompatibility antigen (mHAg). Subsequently, several CTL lines were established by a limiting dilution method and analyzed. One of these CTL lines, 16C12 CTL which used a single TCRβV3S1 for CD8 cells, lysed HLA-A31-positive leukemic cells and EBV-LCL, but not fibroblasts. The cytotoxicity against the patient's leukemic cells and EBV-LCL was blocked by anti-HLA-A31 moAb, anti-HLA-class I moAb, and anti-CD8 moAb, suggesting that this mHAg was presented with HLA-A31. The antigen recognized by 16C12 CTL seemed to be a novel mHAg, since HLA-A31 restricted antigen has not been reported to date and 16C12 CTL showed no cytotoxicity against EBV-LCL which probably express known mHAgs. CTL detecting this mHAg may play an important role in the GVL effect in HLA-A31-positive BMT patients.

元の言語English
ページ(範囲)129-137
ページ数9
ジャーナルBone Marrow Transplantation
24
発行部数2
DOI
出版物ステータスPublished - 01-01-1999

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Minor Histocompatibility Antigens
Cytotoxic T-Lymphocytes
Bone Marrow
Tissue Donors
Lymphocytes
Human Herpesvirus 4
Cell Line
Philadelphia Chromosome
HLA-A31 antigen
Siblings
Fibroblasts
Antigens

All Science Journal Classification (ASJC) codes

  • Hematology
  • Transplantation

これを引用

Yazaki, M. ; Takahashi, T. ; Andho, M. ; Akatsuka, Yoshiki ; Ito, Tetsuya ; Miyake, Y. ; Ito, Y. ; Nakamura, S. ; Wada, Y. / A novel minor histocompatibility antigen recognized by HLA-A31 restricted cytotoxic T lymphocytes generated from HLA-identical bone marrow donor lymphocytes. :: Bone Marrow Transplantation. 1999 ; 巻 24, 番号 2. pp. 129-137.
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A novel minor histocompatibility antigen recognized by HLA-A31 restricted cytotoxic T lymphocytes generated from HLA-identical bone marrow donor lymphocytes. / Yazaki, M.; Takahashi, T.; Andho, M.; Akatsuka, Yoshiki; Ito, Tetsuya; Miyake, Y.; Ito, Y.; Nakamura, S.; Wada, Y.

:: Bone Marrow Transplantation, 巻 24, 番号 2, 01.01.1999, p. 129-137.

研究成果: Article

TY - JOUR

T1 - A novel minor histocompatibility antigen recognized by HLA-A31 restricted cytotoxic T lymphocytes generated from HLA-identical bone marrow donor lymphocytes

AU - Yazaki, M.

AU - Takahashi, T.

AU - Andho, M.

AU - Akatsuka, Yoshiki

AU - Ito, Tetsuya

AU - Miyake, Y.

AU - Ito, Y.

AU - Nakamura, S.

AU - Wada, Y.

PY - 1999/1/1

Y1 - 1999/1/1

N2 - Bulk cytotoxic T lymphocytes (CTL) were generated by in vitro stimulation of BMT donor lymphocytes with Philadelphia chromosome (Ph)-positive leukemic cells from an HLA-identical sibling patient. CTL were cytotoxic against the patient's leukemic cells as well as the EBV-lymphoblastoid cell line (EBV-LCL) generated from the patient's cells, suggesting that they recognize a minor histocompatibility antigen (mHAg). Subsequently, several CTL lines were established by a limiting dilution method and analyzed. One of these CTL lines, 16C12 CTL which used a single TCRβV3S1 for CD8 cells, lysed HLA-A31-positive leukemic cells and EBV-LCL, but not fibroblasts. The cytotoxicity against the patient's leukemic cells and EBV-LCL was blocked by anti-HLA-A31 moAb, anti-HLA-class I moAb, and anti-CD8 moAb, suggesting that this mHAg was presented with HLA-A31. The antigen recognized by 16C12 CTL seemed to be a novel mHAg, since HLA-A31 restricted antigen has not been reported to date and 16C12 CTL showed no cytotoxicity against EBV-LCL which probably express known mHAgs. CTL detecting this mHAg may play an important role in the GVL effect in HLA-A31-positive BMT patients.

AB - Bulk cytotoxic T lymphocytes (CTL) were generated by in vitro stimulation of BMT donor lymphocytes with Philadelphia chromosome (Ph)-positive leukemic cells from an HLA-identical sibling patient. CTL were cytotoxic against the patient's leukemic cells as well as the EBV-lymphoblastoid cell line (EBV-LCL) generated from the patient's cells, suggesting that they recognize a minor histocompatibility antigen (mHAg). Subsequently, several CTL lines were established by a limiting dilution method and analyzed. One of these CTL lines, 16C12 CTL which used a single TCRβV3S1 for CD8 cells, lysed HLA-A31-positive leukemic cells and EBV-LCL, but not fibroblasts. The cytotoxicity against the patient's leukemic cells and EBV-LCL was blocked by anti-HLA-A31 moAb, anti-HLA-class I moAb, and anti-CD8 moAb, suggesting that this mHAg was presented with HLA-A31. The antigen recognized by 16C12 CTL seemed to be a novel mHAg, since HLA-A31 restricted antigen has not been reported to date and 16C12 CTL showed no cytotoxicity against EBV-LCL which probably express known mHAgs. CTL detecting this mHAg may play an important role in the GVL effect in HLA-A31-positive BMT patients.

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