A novel nuclear phosphoprotein, GANP, is up-regulated in centrocytes of the germinal center and associated with MCM3, a protein essential for DNA replication

Kazuhiko Kuwahara, Mikoto Yoshida, Eisaku Kondo, Atsuko Sakata, Yuko Watanabe, Eiji Abe, Yoshihiko Kouno, Shinjirou Tomiyasu, Satoru Fujimura, Takeshi Tokuhisa, Hiroshi Kimura, Taichi Ezaki, Nobuo Sakaguchi

研究成果: Article査読

59 被引用数 (Scopus)

抄録

Antigen (Ag) immunization induces formation of the germinal center (GC), with large, rapidly proliferating centroblasts in the dark zone, and small, nondividing centrocytes in the light zone. We identified a novel nuclear protein, GANP, that is up-regulated in centrocytes. We found that GANP was up-regulated in GC B cells of Peyer's patches in normal mice and in spleens from Ag-Immunized mice. GANP-positive cells appeared in the light zone of the GC, with coexpression of the peanut agglutinin (PNA) (PNA)-positive B220- positive phenotype. The expression of GANP was strikingly correlated with GC formation because Bcl6-deficient mice did not show the up-regulation of GANP. GANP-positive cells were mostly surrounded by follicular dendritic cells. Stimulation with anti-μ and anti-CD40 induced up-regulation of ganp messenger RNA as well as GANP protein in B220-positive B cells in vitro. GANP is a 210-kd protein localized in both the cytoplasm and nuclei, with a homologous region to Map80 that is associated with MCM3, a protein essential for DNA replication. Remarkably, GANP is associated with MCM3 in B cells and MCM3 is also up-regulated in the GC area. These results suggest that the up- regulation of GANP might participate in the development of Ag-driven B cells in GCs through its interaction with MCM3. (C) 2000 by The American Society of Hematology.

本文言語English
ページ(範囲)2321-2328
ページ数8
ジャーナルBlood
95
7
DOI
出版ステータスPublished - 01-04-2000

All Science Journal Classification (ASJC) codes

  • 生化学
  • 免疫学
  • 血液学
  • 細胞生物学

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