TY - JOUR
T1 - A novel strategy for the negative selection in mouse embryonic stem cells operated with immunotoxin-mediated cell targeting
AU - Kobayashi, Kazuto
AU - Ohye, Tamae
AU - Pastan, Ira
AU - Nagatsu, Toshiharu
N1 - Funding Information:
We thank T. Honjo for human IL-2Rα cDNA, T. Uchiyama for anti-Tac monoclonal antibody, and H. Sawada for technical instruction of immunofluorescence. This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.
PY - 1996
Y1 - 1996
N2 - Immunotoxoin-mediated cell targeting (IMCT) is a technique for conditionally ablating specific cell types based on the cytotoxic activity of a recombinant immunotoxin anti-Tac (Fv)-PE40. To examine the feasibility of this technique for the negative selection in mouse embryonic stem (ES) cells, we investigated the responsiveness of cells expressing human interleukin-2 receptor α subunit to anti-Tac(Fv)-PE40. The immunotoxin treatment efficiently eliminated only ES cells bearing the receptor as a consequence of the target specificity of anti-Tac(Fv)-PE40, indicating that IMCT can be used as a novel strategy for positive and negative selection to enrich ES cell clones with a targeted mutation.
AB - Immunotoxoin-mediated cell targeting (IMCT) is a technique for conditionally ablating specific cell types based on the cytotoxic activity of a recombinant immunotoxin anti-Tac (Fv)-PE40. To examine the feasibility of this technique for the negative selection in mouse embryonic stem (ES) cells, we investigated the responsiveness of cells expressing human interleukin-2 receptor α subunit to anti-Tac(Fv)-PE40. The immunotoxin treatment efficiently eliminated only ES cells bearing the receptor as a consequence of the target specificity of anti-Tac(Fv)-PE40, indicating that IMCT can be used as a novel strategy for positive and negative selection to enrich ES cell clones with a targeted mutation.
UR - https://www.scopus.com/pages/publications/0029816051
UR - https://www.scopus.com/pages/publications/0029816051#tab=citedBy
U2 - 10.1093/nar/24.18.3653
DO - 10.1093/nar/24.18.3653
M3 - Article
C2 - 8836200
AN - SCOPUS:0029816051
SN - 0305-1048
VL - 24
SP - 3653
EP - 3655
JO - Nucleic acids research
JF - Nucleic acids research
IS - 18
ER -