A novel tetracycline-dependent transactivator with E2F4 transcriptional activation domain

Kiwamu Akagi, Masayuki Kanai, Hideyuki Saya, Tomoko Kozu, Anton Berns

研究成果: Article査読

33 被引用数 (Scopus)

抄録

A tetracycline-controlled gene expression system provides a powerful tool to dissect the functions of gene products. However, it often appears difficult to establish cell lines or transgenic animals stably expressing tetracycline-dependent transactivators, possibly as a result of toxicity of the transactivator domains used. In order to overcome this problem, we developed a novel tetracycline-dependent transactivator that works efficiently in mammalian cells. This transactivator is a fusion of the tet reverse repressor mutant and the transcriptional activating domain of human E2F4, which is ubiquitously expressed in vivo. We demonstrate here that this tetracycline-regulated gene expression system provides a two log transcriptional activation in mammalian cells as assessed by northern blot and luciferase analyses. Combining this system with green fluorescent protein reporter systems or microarray gene expression profiling will facilitate the study of gene function.

本文言語English
ページ(範囲)23
ページ数1
ジャーナルNucleic acids research
29
4
DOI
出版ステータスPublished - 15-02-2001
外部発表はい

All Science Journal Classification (ASJC) codes

  • Genetics

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