A prunus mume extract stimulated the proliferation and differentiation of osteoblastic MC3T3-E1 Cells

Ryohei Kono, Yoshiharu Okuno, Ken Ichi Inada, Akihiko Tokuda, Hiroshi Hashizume, Munehito Yoshida, Misa Nakamura, Hirotoshi Utsunomiya

研究成果: ジャーナルへの寄稿学術論文査読

22 被引用数 (Scopus)

抄録

Osteoporosis is a serious disease caused by decreased bone mass. There is constant matrix remodeling in bones, by which bone formation is performed by osteoblastic cells, whereas bone resorption is accomplished by osteoclast cells. We investigated the effect of a Japanese apricot (Prunus mume SIBE. et ZUCC.) extract on the proliferation and osteoblastic differentiation in pre-osteoblastic MC3T3-E1 cells. An alkaline phosphatase (ALP) activity assay, cell proliferation assay, alizarin red staining and expression analysis of osteoblastic genes were carried out to assess the proliferation and osteoblastic differentiation. The water-soluble fraction of Prunus mume (PWF) increased the ALP activity, cell proliferation and mineralization. The gene expression of osteopontin and bone morphogenetic protein-2, which are markers in the early period of osteoblastic differentiation, were significantly enhanced by the PWF treatment. PWF therefore stimulated the proliferation and osteoblastic differentiation of cells and may have potential to prevent osteoporosis.

本文言語英語
ページ(範囲)1907-1911
ページ数5
ジャーナルBioscience, Biotechnology and Biochemistry
75
10
DOI
出版ステータス出版済み - 2011

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 分析化学
  • 生化学
  • 応用微生物学とバイオテクノロジー
  • 分子生物学
  • 有機化学

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