A RING finger motif regulates transforming activity of the rfp/ret fusion gene

Nakaba Hasegawa, Toshihide Iwashita, Naoya Asai, Hideki Murakami, Yosuke Iwata, Takeshi Isomura, Hidemi Goto, Tetsuo Hayakawa, Masahide Takahashi

研究成果: ジャーナルへの寄稿学術論文査読

35 被引用数 (Scopus)

抄録

The rfp/ret transforming gene was generated by DNA rearrangement which occurred during transfection of NIH 3T3 cells. The amino-terminal half of Rfp with a RING finger motif was fused to the truncated Ret receptor tyrosine kinase, leading to a transforming sequence, Rfp/Ret. In the present study, to elucidate the importance of a RING finger motif for its transforming activity, we mutated cysteine or histidine residues present in the motif that are believed to be crucial for the tertiary structure of the protein. Substitution of Cys-16, Cys-31, or His-33 markedly decreased the transforming activity of Rfp/Ret. These mutations also resulted in a striking decrease of tyrosine phosphorylation of the mutant proteins in transfectants. In contrast, mutations of Cys-118 or His-124 in the second cysteine/histidine-rich motif (called B box) of Rfp/Ret did not affect its activity. These results thus indicated that the RING finger structure is critical for the full transforming activity of Rfp/Ret.

本文言語英語
ページ(範囲)627-631
ページ数5
ジャーナルBiochemical and Biophysical Research Communications
225
2
DOI
出版ステータス出版済み - 14-08-1996
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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