TY - JOUR
T1 - A simple cytogenetic method to detect chromosomally integrated human herpesvirus-6
AU - Ohye, Tamae
AU - Kawamura, Yoshiki
AU - Inagaki, Hidehito
AU - Yoshikawa, Akiko
AU - Ihira, Masaru
AU - Yoshikawa, Tetsushi
AU - Kurahashi, Hiroki
N1 - Funding Information:
The authors thank Drs. Koji Kato, Junko Oikawa, Hiroshi Yagasaki, Takahiro Niizuma, Yoshiyuki Takahashi, Seiji Kojima, Takuya Maeda, Tadanobu Yoshikawa, Naomi Kubota, Hisashi Yamamoto, and Akifumi Endo for providing samples, Drs. Makiko Tsutsumi and Takema Kato for helpful discussions, and Junko Kato, Noriko Hayashizono, and Narumi Kamiya for technical assistance. This work was supported by a grant-in-aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan , and from the Ministry of Health, Labour and Welfare of Japan , to H.K.
Publisher Copyright:
© 2015 Elsevier B.V.
PY - 2016/2/1
Y1 - 2016/2/1
N2 - Some healthy individuals carry human herpesvirus-6 (HHV-6) within a host chromosome, which is called inherited chromosomally integrated human herpesvirus-6 (iciHHV-6). Because iciHHV-6 is generally considered a non-pathogenic condition, it is important to distinguish iciHHV-6 from HHV-6 reactivation in immunocompromised hosts because both conditions manifest high copy numbers of the HHV-6 in peripheral blood mononuclear cells. Although fluorescent in situ hybridization (FISH) is a reliable method for the diagnosis of iciHHV-6, HHV-6-specific FISH probes are not commercially available. In our present study, we established a simple PCR-based method for producing FISH probes that can detect the chromosomal integration site of iciHHV-6 at high sensitivity. Using these probes, we confirmed that HHV-6 signals were consistently located at the telomeric region in all of the 13 iciHHV-6 individuals examined. Interestingly, in all seven Japanese iciHHV-6A patients, signals were detected exclusively on chromosome 22q. This method provides a simple and fast approach for iciHHV-6 diagnosis in the clinical laboratory.
AB - Some healthy individuals carry human herpesvirus-6 (HHV-6) within a host chromosome, which is called inherited chromosomally integrated human herpesvirus-6 (iciHHV-6). Because iciHHV-6 is generally considered a non-pathogenic condition, it is important to distinguish iciHHV-6 from HHV-6 reactivation in immunocompromised hosts because both conditions manifest high copy numbers of the HHV-6 in peripheral blood mononuclear cells. Although fluorescent in situ hybridization (FISH) is a reliable method for the diagnosis of iciHHV-6, HHV-6-specific FISH probes are not commercially available. In our present study, we established a simple PCR-based method for producing FISH probes that can detect the chromosomal integration site of iciHHV-6 at high sensitivity. Using these probes, we confirmed that HHV-6 signals were consistently located at the telomeric region in all of the 13 iciHHV-6 individuals examined. Interestingly, in all seven Japanese iciHHV-6A patients, signals were detected exclusively on chromosome 22q. This method provides a simple and fast approach for iciHHV-6 diagnosis in the clinical laboratory.
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U2 - 10.1016/j.jviromet.2015.11.001
DO - 10.1016/j.jviromet.2015.11.001
M3 - Article
C2 - 26549829
AN - SCOPUS:84949521974
VL - 228
SP - 74
EP - 78
JO - Journal of Virological Methods
JF - Journal of Virological Methods
SN - 0166-0934
ER -