A third broad lineage of major histocompatibility complex (MHC) class I in teleost fish; MHC class II linkage and processed genes

J・m Dijkstra, Takayuki Katagiri, Kazuyoshi Hosomichi, Kazuyo Yanagiya, Hidetoshi Inoko, Mitsuru Ototake, Takashi Aoki, Keiichiro Hashimoto, Takashi Shiina

研究成果: Article

36 引用 (Scopus)

抄録

Most of the previously studied teleost MHC class I molecules can be classified into two broad lineages: "U" and "Z/ZE." However, database reports on genes in cyprinid and salmonid fishes show that there is a third major lineage, which lacks detailed analysis so far. We designated this lineage "L" because of an intriguing linkage characteristic. Namely, one zebrafish L locus is closely linked with MHC class II loci, despite the extensively documented nonlinkage of teleost class I with class II. The L lineage consists of highly variable, nonclassical MHC class I genes, and has no apparent orthologues outside teleost fishes. Characteristics that distinguish the L lineage from most other MHC class I are (1) absence of two otherwise highly conserved tryptophan residues W51 and W60 in the α1 domain, (2) a low GC content of the α1 and α2 exons, and (3) an HINLTL motif including a possible glycosylation site in the α3 domain. In rainbow trout (Oncorhynchus mykiss) we analyzed several intact L genes in detail, including their genomic organization and transcription pattern. The gene Onmy-LAA is quite different from the genes Onmy-LBA, Onmy-LCA, Onmy-LDA, and Onmy-LEA, while the latter four are similar and categorized as "Onmy-LBA-like." Whereas the Onmy-LAA gene is organized like a canonical MHC class I gene, the Onmy-LBA-like genes are processed and lack all introns except intron 1. Onmy-LAA is predominantly expressed in the intestine, while the Onmy-LBA-like transcripts display a rather homogeneous tissue distribution. To our knowledge, this is the first description of an MHC class I lineage with multiple copies of processed genes, which are intact and transcribed. The present study significantly improves the knowledge of MHC class I variation in teleosts.

元の言語English
ページ(範囲)305-321
ページ数17
ジャーナルImmunogenetics
59
発行部数4
DOI
出版物ステータスPublished - 01-04-2007

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MHC Class II Genes
Pseudogenes
Major Histocompatibility Complex
Fishes
MHC Class I Genes
Genes
Oncorhynchus mykiss
Introns
Base Composition
Zebrafish
Tissue Distribution
Glycosylation
Tryptophan
Intestines
Exons
Databases

All Science Journal Classification (ASJC) codes

  • Immunology
  • Genetics

これを引用

Dijkstra, J・m ; Katagiri, Takayuki ; Hosomichi, Kazuyoshi ; Yanagiya, Kazuyo ; Inoko, Hidetoshi ; Ototake, Mitsuru ; Aoki, Takashi ; Hashimoto, Keiichiro ; Shiina, Takashi. / A third broad lineage of major histocompatibility complex (MHC) class I in teleost fish; MHC class II linkage and processed genes. :: Immunogenetics. 2007 ; 巻 59, 番号 4. pp. 305-321.
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abstract = "Most of the previously studied teleost MHC class I molecules can be classified into two broad lineages: {"}U{"} and {"}Z/ZE.{"} However, database reports on genes in cyprinid and salmonid fishes show that there is a third major lineage, which lacks detailed analysis so far. We designated this lineage {"}L{"} because of an intriguing linkage characteristic. Namely, one zebrafish L locus is closely linked with MHC class II loci, despite the extensively documented nonlinkage of teleost class I with class II. The L lineage consists of highly variable, nonclassical MHC class I genes, and has no apparent orthologues outside teleost fishes. Characteristics that distinguish the L lineage from most other MHC class I are (1) absence of two otherwise highly conserved tryptophan residues W51 and W60 in the α1 domain, (2) a low GC content of the α1 and α2 exons, and (3) an HINLTL motif including a possible glycosylation site in the α3 domain. In rainbow trout (Oncorhynchus mykiss) we analyzed several intact L genes in detail, including their genomic organization and transcription pattern. The gene Onmy-LAA is quite different from the genes Onmy-LBA, Onmy-LCA, Onmy-LDA, and Onmy-LEA, while the latter four are similar and categorized as {"}Onmy-LBA-like.{"} Whereas the Onmy-LAA gene is organized like a canonical MHC class I gene, the Onmy-LBA-like genes are processed and lack all introns except intron 1. Onmy-LAA is predominantly expressed in the intestine, while the Onmy-LBA-like transcripts display a rather homogeneous tissue distribution. To our knowledge, this is the first description of an MHC class I lineage with multiple copies of processed genes, which are intact and transcribed. The present study significantly improves the knowledge of MHC class I variation in teleosts.",
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A third broad lineage of major histocompatibility complex (MHC) class I in teleost fish; MHC class II linkage and processed genes. / Dijkstra, J・m; Katagiri, Takayuki; Hosomichi, Kazuyoshi; Yanagiya, Kazuyo; Inoko, Hidetoshi; Ototake, Mitsuru; Aoki, Takashi; Hashimoto, Keiichiro; Shiina, Takashi.

:: Immunogenetics, 巻 59, 番号 4, 01.04.2007, p. 305-321.

研究成果: Article

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T1 - A third broad lineage of major histocompatibility complex (MHC) class I in teleost fish; MHC class II linkage and processed genes

AU - Dijkstra, J・m

AU - Katagiri, Takayuki

AU - Hosomichi, Kazuyoshi

AU - Yanagiya, Kazuyo

AU - Inoko, Hidetoshi

AU - Ototake, Mitsuru

AU - Aoki, Takashi

AU - Hashimoto, Keiichiro

AU - Shiina, Takashi

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