Acid sphingomyelinase regulates glucose and lipid metabolism in hepatocytes through AKT activation and AMP-activated protein kinase suppression

Yosuke Osawa, Ekihiro Seki, Yuzo Kodama, Atsushi Suetsugu, Kouichi Miura, Masayuki Adachi, Hiroyasu Ito, Yoshimune Shiratori, Yoshiko Banno, Jerrold M. Olefsky, Masahito Nagaki, Hisataka Moriwaki, David A. Brenner, Mitsuru Seishima

研究成果: ジャーナルへの寄稿学術論文査読

44 被引用数 (Scopus)

抄録

Acid sphingomyelinase (ASM) regulates the homeostasis of sphingolipids, including ceramides and sphingosine-1-phosphate (S1P). Because sphingolipids regulate AKT activation, we investigated the role of ASM in hepatic glucose and lipid metabolism. Initially, we overexpressed ASM in the livers of wild-type and diabetic db/db mice by adenovirus vector (Ad5ASM). In these mice, glucose tolerance was improved, and glycogen and lipid accumulation in the liver were increased. Using primary cultured hepatocytes, we confirmed that ASM increased glucose uptake, glycogen deposition, and lipid accumulation through activation of AKT and glycogen synthase kinase-3(β. In addition, ASM induced up-regulation of glucose transporter 2 accompanied by suppression of AMP-activated protein kinase (AMPK) phosphorylation. Loss of sphingosine kinase-1 (SphK1) diminished ASM-mediated AKT phosphorylation, but exogenous S1P induced AKT activation in hepatocytes. In contrast, SphK1 deficiency did not affect AMPK activation. These results suggest that the SphK/S1P pathway is required for ASM-mediated AKT activation but not for AMPK inactivation. Finally, we found that treatment with high-dose glucose increased glycogen deposition and lipid accumulation in wild-type hepatocytes but not in ASM-cells. This result is consistent with glucose intolerance in ASM-mice. In conclusion, ASM modulates AKT activation and AMPK inactivation, thus regulating glucose and lipid metabolism in the liver.

本文言語英語
ページ(範囲)1133-1144
ページ数12
ジャーナルFASEB Journal
25
4
DOI
出版ステータス出版済み - 04-2011
外部発表はい

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 生化学
  • 分子生物学
  • 遺伝学

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