Active and passive immunization protects against lethal, extreme drug resistant-Acinetobacter baumannii infection

Guanpingshen Luo, Lin Lin, Ashraf S. Ibrahim, Beverlie Baquir, Paul Pantapalangkoor, Robert A. Bonomo, Yohei Doi, Mark D. Adams, Thomas A. Russo, Brad Spellberg

研究成果: ジャーナルへの寄稿学術論文査読

157 被引用数 (Scopus)

抄録

Extreme-drug-resistant (XDR) Acinetobacter baumannii is a rapidly emerging pathogen causing infections with unacceptably high mortality rates due to inadequate available treatment. New methods to prevent and treat such infections are a critical unmet medical need. To conduct a rational vaccine discovery program, OmpA was identified as the primary target of humoral immune response after intravenous infection by A. baumannii in mice. OmpA was >99% conserved at the amino acid level across clinical isolates harvested between 1951 and 2009 from cerebrospinal fluid, blood, lung, and wound infections, including carbapenem-resistant isolates, and was ≥89% conserved among other sequenced strains, but had minimal homology to the human proteome. Vaccination of diabetic mice with recombinant OmpA (rOmpA) with aluminum hydroxide adjuvant markedly improved survival and reduced tissue bacterial burden in mice infected intravenously. Vaccination induced high titers of anti-OmpA antibodies, the levels of which correlated with survival in mice. Passive transfer with immune sera recapitulated protection. Immune sera did not enhance complement-mediated killing but did enhance opsonophagocytic killing of A. baumannii. These results define active and passive immunization strategies to prevent and treat highly lethal, XDR A. baumannii infections.

本文言語英語
論文番号e29446
ジャーナルPloS one
7
1
DOI
出版ステータス出版済み - 10-01-2012
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学、遺伝学、分子生物学一般
  • 農業および生物科学一般
  • 一般

フィンガープリント

「Active and passive immunization protects against lethal, extreme drug resistant-Acinetobacter baumannii infection」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル